Open Access
ARTICLE
In Vitro Propagation, Isolation and Expression Studies of Suaeda edulis Genes Involved in the Osmoprotectants Biosynthesis
Gloria Viviana Cerrillo-Rojas1, Mariana Tiscareño-Andrade1, Ana Erika Ochoa-Alfaro2, Eugenio Pérez-Molphe Balch1, Ruth Elena Soria-Guerra2, José Francisco Morales-Domínguez1,*
1 Departamento de Química, Universidad Autónoma de Aguascalientes, Centro de Ciencias Básicas, Ciudad Universitaria, Aguascalientes, 20131, México
2 Facultad de Química, Universidad Autónoma de San Luis Potosí, Zona Universitaria, San Luis Potosí, 78210, México
* Corresponding Author: José Francisco Morales-Domínguez. Email:
Phyton-International Journal of Experimental Botany 2020, 89(3), 715-726. https://doi.org/10.32604/phyton.2020.09769
Received 20 January 2020; Accepted 20 February 2020; Issue published 22 June 2020
Abstract
Halophytes are an excellent choice for the study of genes conferring
salt tolerance to salt-sensitive plants and, they are suitable for reclamation and
remediation of saline soil. We develop an
in vitro plant propagation protocol
and studies of genes involved with GB and Pro biosynthesis in Suaeda edulis.
Axillary buds were used as explants and cultured in different treatments on Murashige and Skoog (MS) medium supplemented with different concentrations and
combinations of plant growth regulators. The highest number of multiple shoots
was on MS medium containing 1 mg/L Benzyladenine (BA) and / or 2 g/L activated carbon with 5.5 ± 06 shoots per explant. The identification and expression
analysis of genes involved in glycine betaine (GB) biosynthesis were S-adenosylmethionine synthetase (
SAMS), choline monooxygenase (
CMO) and betaine aldehyde dehydrogenase (
BADH), and for proline (Pro) was pyrroline 5-carboxylate
synthetase (
P5CS). These sequences shared 90–95% of identity with others plant
homologous in public databases. The amino acids sequence analysis showed that
all these peptides contain some of the conserved motifs of those kinds of
enzymes. The qRT-PCR analysis revealed a higher expression of
SeBADH,
SeCMO, and,
SeP5CS genes in the roots and leaves from plants collected in
the field in contrast with from
in vitro plants. However, the expression level of
SeSAMS was higher only in the leaves of plants collected in the field when compared to those cultivated
in vitro.
Keywords
Cite This Article
Cerrillo-Rojas, G. V., Tiscareño-Andrade, M., Ochoa-Alfaro, A. E., Balch, E. P., Soria-Guerra, R. E. et al. (2020).
In Vitro Propagation, Isolation and Expression Studies of
Suaeda edulis Genes Involved in the Osmoprotectants Biosynthesis.
Phyton-International Journal of Experimental Botany, 89(3), 715–726.