Open Access
ARTICLE
Effect of microRNA-143-3p-mediated CTNND1 on the biological function of lung cancer cells
Xinxiong FEI, Wenbin HU, Gangsheng WANG, Chunyan SU, Xuqun HUANG, Zhongjun JIANG*
Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Department of Internal Medicine-Oncology, Huangshi, 435200, China
* Address correspondence to: Zhongjun Jiang,
BIOCELL 2020, 44(1), 81-88. https://doi.org/10.32604/biocell.2020.08125
Issue published 01 March 2020
Abstract
Lung cancer poses a serious threat to human life with high incidence and miRNA is an important biomarker
in tumors. This study aimed to explore the effect of miR-143-3p on the biological function of lung cancer cells and the
underlying mechanism. Eighty-seven samples of lung cancer tissues and 81 samples of tumor-adjacent tissues from
patients undergoing radical lung cancer surgery in our hospital were collected. The lung cancer cells and lung fibroblast
cells (HFL-1) were purchased, and then miR-143-3p-mimics, miR-NC, si-CTNND1, and NC were transfected into
A549 and PC-9 cells to establish cell models. MiR-143-3p and CTNND1 expression levels were measured by the qRTPCR,
Bax, Bcl-2, and CTNND1 expression levels by the Western Blot (WB), and cell proliferation, invasion, and
apoptosis by the MTT assay, Transwell assay, and flow cytometry. Dual luciferase report assay was used to determine
the relationship between miR-143-3p and CTNND1. In this study, miR-143-3p was lowly expressed in lung cancer
and CTNND1 was highly expressed in lung cancer. The overexpression of miR-143-3p inhibited cell proliferation
and invasion, promoted cell apoptosis, significantly increased Bax protein expression, and decreased Bcl-2 protein
expression. The inhibition of CTNND1 led to opposite biological characteristic in cells. The dual luciferase reporter
assay demonstrated that miR-143-3p was a target region of CTNND1. Such results suggest that miR-143-3p can inhibit
the proliferation and invasion of lung cancer cells by regulating the expression of CTNND1 and promote the apoptosis
of lung cancer cells, sott is expected to be a potential target for lung cancer.
Keywords
Cite This Article
FEI,, X. (2020). Effect of microRNA-143-3p-mediated CTNND1 on the biological function of lung cancer cells.
BIOCELL, 44(1), 81–88.
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