TY - EJOU AU - NA, EUN AU - CHOI, MYOUNGWON AU - PARK, INDAL AU - LIM, SUNYOUNG TI - Effect of Black Sea bream extracts on cytokine production in lipopolysaccharide-induced inflammation T2 - BIOCELL PY - 2020 VL - 44 IS - 2 SN - 1667-5746 AB - We investigated the effect of black sea bream extracts on changes in pro-inflammatory and anti-inflammatory cytokines induced by lipopolysaccharide (LPS) in murine splenocytes. The levels of pro-inflammatory cytokines [interleukin (IL)-6, tumor necrosis factor-α (TNF-α), IL-12/IL-23(p40) and IL-17A] and anti-inflammatory cytokines [IL-4, IL-10 and interferon-γ (IFN-γ)] were assessed. Incubation of murine splenocytes with acetone+methylene chloride (A+M) and methanol (MeOH) extracts significantly decreased LPS-induced IL-6, IL-12/IL-23(p40) and IL-17A productions after 6 h incubation (p < 0.05). The A+M and MeOH extracts significantly increased LPS-induced IL-4 and IFN-γ productions at 48 and 72 h incubation (p < 0.05). Treatment with A+M extract resulted in significantly higher IL-10 production in splenocytes after 72 h (p < 0.05). Conclusively, black sea bream extracts were shown to be efficient in falling several pro-inflammatory cytokines while rising anti-inflammatory cytokines. Thus our results suggest that black sea bream extracts selectivity modulate immune events. KW - Black Sea bream KW - Cytokines KW - Interleukin-4 KW - Interleukin-6 KW - Interleukin-17A DO - 10.32604/biocell.2020.08648