||MCB: Molecular & Cellular Biomechanics, Vol. 1, No. 3, pp. 211-220, 2004
||Full length paper in PDF format. Size = 478,348 bytes
||Cantilevers, array, DNA hybridization, protein interaction, multiplexing.
||Microchips;ontaining arrays of cantilever beams have been used to mechanically detect and quantitatively analyze multiple reactions of DNA hybridization and antigen-antibody binding simultaneously. The reaction-induced deflection of a cantilever beam reflects the interplay between strain energy increase of the beam and the free energy reduction of a reaction, providing an ideal tool for investigating the connection between mechanics and chemistry of biomolecular reactions. Since free energy reduction is common for all reactions, the cantilever array forms a universal platform for label-free detection of various specific biomolecular reactions. A few such reactions and their implications in biology and biotechnology are discussed.