Cantilever Arrays for Multiplexed Mechanical Analysis of Biomolecular Reactions
Min Yue; Jeanne C. Stachowiak$^1, \tmspace + \thinmuskip {.1667em}$; and Arunava Majumdar$^1, \tmspace + \thinmuskip {.1667em}$

doi:10.3970/mcb.2004.001.211
Source MCB: Molecular & Cellular Biomechanics, Vol. 1, No. 3, pp. 211-220, 2004
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Keywords Cantilevers, array, DNA hybridization, protein interaction, multiplexing.
Abstract Microchips;ontaining arrays of cantilever beams have been used to mechanically detect and quantitatively analyze multiple reactions of DNA hybridization and antigen-antibody binding simultaneously. The reaction-induced deflection of a cantilever beam reflects the interplay between strain energy increase of the beam and the free energy reduction of a reaction, providing an ideal tool for investigating the connection between mechanics and chemistry of biomolecular reactions. Since free energy reduction is common for all reactions, the cantilever array forms a universal platform for label-free detection of various specific biomolecular reactions. A few such reactions and their implications in biology and biotechnology are discussed.
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