TY - EJOU AU - JJ, Quezada-Rivera AU - Soria-Guerra, RE AU - Pérez-Juárez, FS AU - Martínez-González, L AU - Rodríguez, SE Valdés- AU - Vasco-Méndez, NL AU - Morales-Domínguez, JF TI - Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis T2 - Phyton-International Journal of Experimental Botany PY - 2019 VL - 88 IS - 4 SN - 1851-5657 AB - The use of antimicrobial peptides (AMPs) synthesized by bacteria (bacteriocins) is an alternative for combating multidrug resistant bacterial strains and their production by recombinant route is a viable option for their mass production. The bacteriocin E-760 isolated from the genus Enterococcus sp. has been shown to possess inhibitory activity against Gram-negative and Gram-positive bacteria. In this study, the expression of a chimeric protein coding for E-760 in the nucleus of C. reinhardtii was evaluated, as well as, its antibacterial activity. The synthetic gene E-760S was inserted into the genome of C. reinhardtii using Agrobacterium tumefaciens. A transgenic line was identified in TAP medium with hygromycin and also by PCR. The increment in the culture medium temperature of the transgenic strain at 35 °C for 10 minutes, increased the production level of the recombinant protein from 0.14 (Noninduced culture, NIC) to 0.36% (Induced culture, IC) of total soluble proteins (TSP); this was quantified by an ELISA assay. Recombinant E-760 possesses activity against Staphylococcus aureus in 0.34 U log, Streptococcus agalactiae in 0.48 U log, Enterococcus faecium in 0.36 U log, Pseudomonas aeruginosa in 2 U log and for Klebsiella pneumoniae, the activity was 0.07 U log. These results demonstrate that the nucleus transformation of C. reinhardtii can function as a stable expression platform for the production of the synthetic gene E-760 and it can potentially be used as an antibacterial agent. KW - Heterologous expression; Antibacterial activity; Bacteriocin E-760; Chlamydomonas reinhardtii; Log inactivation; Nuclear transformation DO - 10.32604/phyton.2019.04549