TY  - EJOU
AU  - Jacobs, Bruce L. 
AU  - Smaldone, Marc C. 
AU  - Tyagi, Vikas 
AU  - Philips, Brian J. 
AU  - Jackman, Stephen V. 
AU  - Leng, Wendy W. 
AU  - Tyagi, Pradeep 

TI  - Increased nerve growth factor in neurogenic overactive bladder and interstitial cystitis patients
T2  - Canadian Journal of Urology

PY  - 2010
VL  - 17
IS  - 1
SN  - 1488-5581

AB  - <b>Objectives:</b> Studies have suggested that pathology of the lower urinary tract can be detected by following changes in urinary proteins. We evaluated urine nerve growth factor (NGF) levels from patients with a variety of urologic conditions to examine NGF's role as a future biomarker.<br/>

<b>Materials and methods:</b> Urine samples were obtained from 72 patients with normal non-diseased urinary tracts (n=13), neurogenic overactive bladder (NOAB) (n=13), idiopathic overactive bladder (OAB) (n=17), interstitial cystitis/painful bladder syndrome (IC/PBS) (n=8), prostate cancer (n=7), history of prostate cancer status post robot-assisted laparoscopic prostatectomy (RALP) (n=6), active bladder cancer (n=4), and nephrolithiasis (n=4). Urinary NGF levels were measured by enzyme linked immunosorbent assay (ELISA) using the Emax ImmunoAssay System (Promega, Madison, WI, USA); each NGF level was normalized to the patient's urine creatinine (Cr) level. The Bonferroni correction was used to adjust for multiple comparisons.<br/>

<b>Results:</b> Urinary NGF/Cr levels were significantly elevated in patients with NOAB (23.02 pg/mg (0-293), p=0.004) and IC/PBS (31.24 pg/mg (0-291), p=0.006); and approached significance in patients with nephrolithiasis (19.46 pg/mg (0-85), p=0.06) compared to controls (0.00 pg/mg (0-12).<br/>

<b>Conclusions:</b> Urinary NGF levels were significantly elevated in patients with NOAB and IC/PBS. Future studies are needed to further examine the significance of urinary NGF levels in the pathogenesis of a variety of urologic diseases and whether NGF could be used as a diagnostic or prognostic marker for specific urologic diseases.
KW  - interstitial cystitis/painful bladder syndrome
KW  -  neurogenic overactive bladder
KW  -  nerve growth factor
KW  -  urine
KW  -  biomarker

DO  -