TY  - EJOU
AU  - Manach, Quentin 
AU  - Cussenot, Olivier 
AU  - Rouprêt, Morgan 
AU  - Gamé, Xavier 
AU  - Chartier-Kastler, Emmanuel 
AU  - Reus, Christine 
AU  - Camparo, Philippe 
AU  - Compérat, Eva 
AU  - Phé, Véronique 

TI  - Analysis of bladder cancer subtypes in neurogenic bladder tumors
T2  - Canadian Journal of Urology

PY  - 2018
VL  - 25
IS  - 1
SN  - 1488-5581

AB  - <b>Introduction:</b> To determine whether the validated tumor biomarkers of luminal and basal subtypes of bladder cancer, established in non neuro-urological patients, are applicable to a neuro-urological population.<br/>

<b>Materials and methods:</b> We collected bladder cancer samples from neuro-urological patients (n = 20) and non-neurological controls (n = 40). The expression levels of GATA3 and CK5/6 were analyzed using immunohistochemistry on tissue microarray sections. We also evaluated the correlation between previously identified biomarker expression, gender, age, tumor stage (non-muscle-invasive bladder cancer [NMIBC] vs. muscle-invasive bladder cancer [MIBC]), squamous-cell differentiation, and luminal/basal subtypes using Pearson’s correlation coefficient (r).<br/>

<b>Results:</b> The mean age at diagnosis of bladder cancer in neuro-urological patients was 53.2 years (range: 41–73). Muscle-invasive bladder cancer (MIBC) was observed in 13 neuro-urological patients (65%). The luminal subtype was identified in 7 samples (35%), all showing urothelial differentiation. The basal subtype was found in 13 samples (65%): 12 with squamous-cell differentiation and 1 with sarcomatoid differentiation. GATA3 and CK5/6 were co-expressed in only 6 (30%) neuro-urological patients. A significant positive correlation was observed between GATA3 expression and the luminal subtype (p = 0.00001, r = 0.5676), but not with the neuro-urological status (r = -0.307). Poor correlations were found between CK5/6 expression and neuro-urological status (r = 0.471 and -0.471), squamous-cell differentiation (r = 0.092), tumor stage NMIBC/MIBC (r = -0.118 and 0.118), and luminal/basal subtypes (r = -0.157 and 0.194).<br/>

<b>Conclusion:</b> In summary, the expression patterns of GATA3 and CK5/6 were unable to reliably distinguish luminal and basal subtypes of bladder cancer in neuro-urological patients. This suggests that the histopathological signature of bladder cancer in this population may differ from that in non-neuro-urological patients. Additional molecular pathways may be involved in the urothelial carcinogenesis mechanism in neuro-urological individuals.
KW  - basal
KW  -  neurogenic bladder
KW  -  urothelial carcinoma
KW  -  bladder cancer
KW  -  luminal

DO  -