@Article{,
AUTHOR = {Paul H. Chung, Joon Yau Leong, Seth Teplitsky, Patrick J. Shenot, Akhil K. Das, Leonard G. Gomella},
TITLE = {Next-generation DNA sequencing for infected genitourinary implants: How I do it},
JOURNAL = {Canadian Journal of Urology},
VOLUME = {27},
YEAR = {2020},
NUMBER = {5},
PAGES = {10418--10423},
URL = {http://www.techscience.com/CJU/v27n5/60373},
ISSN = {1488-5581},
ABSTRACT = {Infection of artificial urinary sphincters or inflatable penile 
prostheses is one of the most devastating complications 
after prosthetic surgery and can have a significant impact 
on a quality of life. Patients undergoing revision surgery 
with or without device replacement may have increased 
risk for infection when compared to initial primary 
surgery. As such, surgeons may utilize traditional culture 
results to direct antimicrobial therapy for these patients. 
Unfortunately, culture results can be inconclusive in up to one-third of the time even in the setting of active 
device infection. Next-generation sequencing (NGS) of 
DNA is an emerging technology capable of sequencing 
entire bacterial genomes and has the potential to identify 
microbial composition in explanted devices. Herein, we 
describe our institutional experience on NGS utilization 
in patients with genitourinary prostheses. We also 
highlight our methods and techniques to inform readers 
on the potential practices that can enhance the utility and 
diagnostic yield of this new and upcoming technology.},
DOI = {}
}