@Article{,
AUTHOR = {Luca Campedel, Thomas Seisen, Justine Varinot, Géraldine Cancel-Tassin, Alain Ruffion, Emilien Seizilles De Mazancourt, Myriam Decaussin-Petrucci, Grégoire Robert, Nam-Son Vuong, Magali Philipp, Eva Compérat, Morgan Rouprêt, Olivier Cussenot},
TITLE = {Standardizing immunohistochemistry methodology for evaluation of PD-1 and PDL-1 expression in upper tract urothelial  carcinoma},
JOURNAL = {Canadian Journal of Urology},
VOLUME = {28},
YEAR = {2021},
NUMBER = {3},
PAGES = {10719--10724},
URL = {http://www.techscience.com/CJU/v28n3/60322},
ISSN = {1488-5581},
ABSTRACT = {<b>Introduction:</b> Controversy regarding the prognostic and/or predictive role of PD-1 and PD-L1 expression for upper tract urothelial carcinoma (UTUC) could partly be explained by inconsistencies in the immunohistochemistry (IHC) methodology. The objective is to standardize the methodology for routine evaluation of PD-1 and PD-L1 expression in UTUC patients.<br/>
<b>Materials and methods:</b> Twenty-two cases treated with radical nephroureterectomy between 1996 and 2015 at 11 French hospitals were randomly selected to compare different methodologies for evaluation of PD-1 and PD-L1 expression. IHC was carried out on whole tissue sections and 0.6 mm- or 2 mm-core tissue micro-arrays (TMAs) using PD-1 NAT105 and PD-L1 28.8 or E1L3N on both tumor cells and tumor-infiltrating immune cells (TILs). Results obtained with whole tissue sections (WTS) were compared to those obtained with 0.6 mm- and 2 mm-core TMAs. Concordance was evaluated using Kappa coefficient.<br/>
<b>Results:</b> For evaluation of PD-1 and PD-L1 expression, the best concordance with WTS was observed using the PD-1 NAT105 and PD-L1 28.8 antibody on 2 mm-core TMAs, with a 5% cut-off for positivity on TILs and tumor cells, respectively (Kappa = 0.8).<br/>
<b>Conclusions:</b> The most accurate methodology for routine evaluation of PD-1 and PD-L1 expression in UTUC may be based on 2 mm-core TMAs using NAT105 and 28.8 antibodies with a 5% cut-off for positivity on TILs and tumor cells, respectively.},
DOI = {}
}