@Article{CL.2025.226.507,
AUTHOR = {C. B. Cui, G. C. Yang, Z. Zhang, X. J. Wang},
TITLE = {Synthesis of MoS<sub>2</sub>/Fe<sub>3</sub>O<sub>4</sub> composites for the detection of liver cancer biomarker alpha-fetoprotein},
JOURNAL = {Chalcogenide Letters},
VOLUME = {22},
YEAR = {2025},
NUMBER = {6},
PAGES = {507--520},
URL = {http://www.techscience.com/CL/v22n6/64852},
ISSN = {1584-8663},
ABSTRACT = {This research introduces an innovative aptasensor for detecting alpha-fetoprotein with 
exceptional sensitivity and specificity, employing a novel MoS<sub>2</sub>/Fe<sub>3</sub>O<sub>4</sub> composite fabricated 
through an advanced in-situ growth methodology. The composite exhibited a hierarchical 
flower-like structure with uniformly distributed Fe3O4 nanoparticles, confirmed by SEM, 
XRD, and Raman spectroscopy. The MoS<sub>2</sub>/Fe<sub>3</sub>O<sub>4</sub> composite demonstrated a 66% increase 
in surface area (7.16 m<sup>²</sup>/g) compared to pristine MoS2, enhancing aptamer immobilization 
and electron transfer efficiency. Electrochemical characterization revealed a significant 
increase in interfacial resistance upon AFP binding, with a detection limit of 0.3 pg/mL and 
a dual linear range of 0.001–0.1 ng/mL and 0.1–100 ng/mL. The aptasensor showed 
excellent specificity, negligible interference from proteins such as CEA and PSA, and high 
reproducibility (RSD < 4.2%). Stability tests indicated 95% signal retention after 7 days and 
87% after 30 days. Real sample analysis in spiked human serum achieved recovery rates of 
96.8–102.3%. These findings underscore the potential of the MoS2/Fe3O4-based aptasensor 
as a cost-effective, reliable tool for early liver cancer diagnosis, with significant 
improvements in sensitivity and practicality over conventional methods. },
DOI = {10.15251/CL.2025.226.507}
}