TY  - EJOU
AU  - Klausz, Gergely 
AU  - Tiszai, Andrea 
AU  - Tiszlavicz, László 
AU  - Gyulai, Zsófia 
AU  - Lénárt, Zsuzsa 
AU  - Lonovics, János 
AU  - Mándi, Yvette 

TI  - Local and peripheral cytokine response and CagA status of <i>Helicobacter pylori</i>-positive patients with duodenal ulcer
T2  - European Cytokine Network

PY  - 2003
VL  - 14
IS  - 3
SN  - 1952-4005

AB  - The mucosal production of TNF-α, IL-6, IL-8, IL-10 and nitrotyrosine was investigated in
<i>H. pylori</i>-positive patients with duodenal ulcer (DU). The concentrations of these cytokines in gastric antrum
mucosal specimens from patients infected with <i>H. pylori</i> (n = 40) were determined by ELISA and compared with
data on mucosal specimens from <i>H. pylori</i>-negative patients (n = 12). Nitrotyrosine was determined by ECL
Western blotting. It was additionally investigated whether the tissue levels of the cytokines correlated with the
peripheral cytokine levels, and the CagA status of the patients.<br/>
The local TNF-α, IL-6 and IL-8 concentrations in the antral biopsy samples were significantly higher (p < 0.001)
in the patients infected with <i>H. pylori</i> than in the samples from the <i>H. pylori</i>-negative subjects. There was a
negative correlation between the TNF-α and IL-10 concentrations. Further more, in 23 of the 40 biopsy specimens,
considerable nitrotyrosine production was detected by ECL Western blotting. There was no significant difference
in peripheral TNF-α and IL-6 production between the DU patients and healthy blood donors (n = 100; 58% of
whom were also <i>H. pylori</i>-positive). Only the in vitro IL-8-producing capacity was higher in the peripheral blood
of the DU group after ex vivo induction with <i>H. pylori</i>.<br/>
CagA positivity was demonstrated in 39 (97.5%) of the 40 patients with DU, and in 41 (70.7%) of the 58
<i>H. pylori</i>-positive, healthy blood donors.<br/>
This study suggests that besides the bacterial virulence factor, the host response, with an increased mucosal
production of inflammatory cytokines and reactive oxygen and nitrogen species could be relevant to the gastric
pathophysiology in <i>H. pylori</i>-induced DU. There is no generalized cytokine overproduction in these DU patients,
but the moderate increase in in vitro IL-8 production might be of pathophysiological importance.
KW  - <i>H. pylori</i>
KW  -  cytokine
KW  -  nitrotyrosine
KW  -  CagA
KW  -  ELISA
KW  -  Western blot

DO  -