@Article{,
AUTHOR = {Mahmoud Huleihel, Alaa Amash, Olga Sapir, Ester Maor, Sharon Levy, Miriam Katz, Doron Dukler, Lesly Myatt, Gershon Holcberg},
TITLE = {Lipopolysaccharide induces the expression of interleukin-1α distinctly in different compartments of term and preterm human placentae},
JOURNAL = {European Cytokine Network},
VOLUME = {15},
YEAR = {2004},
NUMBER = {1},
PAGES = {30--36},
URL = {http://www.techscience.com/ECN/v15n1/66499},
ISSN = {1952-4005},
ABSTRACT = {The aim of the study was to investigate the stimulatory effect of lipopolysaccharide (LPS) on IL-1α
production in different compartments of term and preterm placental tissues. Homogenates from amnion, chorion,
and from fetal (subchorionic placental tissues, maternal decidua, and mid-placental tissue before and after
perfusion of isolated placental cotyledons of 5 term placentas and 4 placentas obtained after preterm birth
(28-34 W of gestation) were examined. Isolated placental cotyledons were dually perfused LPS (100 ng/kg
perfused placental tissue) was perfused into the maternal side during 10 hours. Homogenates of the samples were
examined by ELISA for IL-1a levels, and paraffin sections of the samples were stained by immunohistochemical
staining, to characterize the cellular origin of placental IL-1α. Paired t test and ANOVA determined statistical
significance. In the homogenates, there was a tendency towards higher IL-1α levels in all preterm placental
compartments as compared to the term compartments before perfusion. A significant increase was observed only
in the chorion compartment (p = 0.035). LPS had significantly increased IL-1α levels only in the decidua
compartment of term placentas as compared to other placental compartments (p = 0.0004), and had decreased
IL-1α levels in the mid-placenta (p = 0.034). In preterm placentas, addition of LPS did not affect the expression
levels of IL-1α in either fetal or maternal compartments as determined by ELISA and immunohistochemical
staining. IL-1α levels in the chorion compartment of preterm placenta were significantly higher as compared to
term placenta. LPS affects placental tissues of term and preterm placentas differently. Also, in the term placentas,
LPS affected the different compartments differently. Thus, IL-1α may have a key role (as a autocrine/paracrine
factor) in the regulation of normal and pathological pregnancy and parturition.},
DOI = {}
}