TY  - EJOU
AU  - Rieth, Horst 
AU  - Mörmann, Maik 
AU  - Luty, Adriana J. F. 
AU  - Assohou-Luty, Constance A. 
AU  - Roupelieva, Maria 
AU  - Kremsner, Peter G. 
AU  - Kube, Dieter 

TI  - A three base pair gene variation within the distal 5’-flanking region of the<i> interleukin-10 (IL-10)</i> gene is related to the in vitro IL-10 production capacity of lipopolysaccharide-stimulated peripheral blood mononuclear cells
T2  - European Cytokine Network

PY  - 2004
VL  - 15
IS  - 2
SN  - 1952-4005

AB  - Interleukin-10 (IL-10) is an important multifunctional immunmodulator. There is evidence that
IL-10 secretion is associated with certain genetic elements of the proximal IL-10 gene 5’-flanking region. The
allelic and genotypic comparison of IL-10 expression by lipopolysaccharide (LPS)- stimulated leukocytes (PBMC)
with a recently discovered distal “indel” DNA-sequence variation at – 7400 bp revealed significant inter-individual
differences in the IL-10 in vitro production capacity. Homozygotes lacking the three base pairs “GGA” (– 7400del)
at this gene locus are characterised by high expression of IL-10 with a median of 1690pg/ml (P ≤ 0.009). The allelic
comparison supports this finding (P ≤ 0.002). Further analysis of the haplotype – 7400/– 1087 showed that
homozygotes for – 7400del/– 1087G may be classified as very strong IL-10 responders with a median IL-10
secretion of 2378 pg/ml (P ≤ 0.025). When leukocytes were stimulated in vitro by dibutyryl-cAMP or infected with
Epstein-Barr virus no significant inter-individual differences between the – 7400indel alleles or genotypes and the
IL-10 in vitro production capacity were observed. Our findings further the understanding of the complexity of
IL-10 gene regulation in relation to defined regulatory gene variations.
KW  - EBV
KW  -  LPS
KW  -  cAMP
KW  -  interleukin-10
KW  -  – 7400indel

DO  -