@Article{,
AUTHOR = {George Fildissis, Kyriaki Venetsanou, Pavlos Myrianthefs, Stylianos Karatzas, Vasilios Zidianakis, George Baltopoulos},
TITLE = {Whole blood pro-inflammatory cytokines and adhesion molecules post-lipopolysaccharides exposure in hyperbaric conditions},
JOURNAL = {European Cytokine Network},
VOLUME = {15},
YEAR = {2004},
NUMBER = {3},
PAGES = {217--221},
URL = {http://www.techscience.com/ECN/v15n3/66377},
ISSN = {1952-4005},
ABSTRACT = {Hyperbaric oxygen (HBO) is a therapeutic intervention with applications in a large variety of
diseases, including traumatic injuries and acute or chronic infections. The presence of pro-inflammatory cytokines
regulates certain factors including adhesion molecules, which play a significant role in HBO effects.<br/>
We have investigated the effect of HBO on pro-inflammatory cytokine release [tumor necrosis factor-α (TNF-α),
interleukin 6 and 8 (IL-6 and IL-8)], and the regulation of adhesion molecules [soluble intercellular adhesion
molecule-1 (sICAM-1) and soluble vascular adhesion molecule (sVCAM)] after lipopolysaccharide (LPS)
stimulation in 16 healthy individuals, originating from an urban area. A total number of 64 samples were treated,
divided into four groups: Group A: not stimulated with LPS and not exposed to HBO. Group B: stimulated with
LPS and not exposed to HBO. Group C: not stimulated with LPS and exposed to HBO. Group D: stimulated with
LPS and exposed to HBO. The LPS stimulation dose was 100 pg/ml for 0.1ml whole blood diluted 1:10. After
incubation, samples were exposed to HBO with 100% O<sub>2</sub> at 2.4 atmospheres absolute (ATA) for 90 min. TNF-α,
IL-6, IL-8 and sICAM-1, sVCAM levels were determined in culture supernatant, with ELISA. We observed an
enhanced effect of LPS stimulation following exposure to HBO, which caused an increase in cytokine production
(TNF-α, IL-6, IL-8), a reduction in sICAM, and no change to sVCAM, while their levels without stimulation
remained almost invariable. The decrease in sICAM levels could be related to the increased levels of IL-8, as the
production of this chemokine is involved in the regulation of adhesion molecules.},
DOI = {}
}