@Article{,
AUTHOR = {Diana Bugarski, Aleksandra Krstić, Marija Vlaški, Marijana Petakov, Gordana Jovčić, Nevenka Stojanović, Pavel Milenkovic},
TITLE = {Interleukine-17-induced inhibitory effect on late stage murine erythroid bone marrow progenitors},
JOURNAL = {European Cytokine Network},
VOLUME = {15},
YEAR = {2004},
NUMBER = {3},
PAGES = {247--254},
URL = {http://www.techscience.com/ECN/v15n3/66381},
ISSN = {1952-4005},
ABSTRACT = {Recent studies have shown that the T cell-derived cytokine, interleukin-17 (IL-17), stimulates
hematopoiesis, specifically granulopoiesis inducing expansion of committed and immature progenitors in bone
marrow. Our previous results pointed to its role in erythropoiesis too, demonstrating significant stimulation of
BFU-E and suppression of CFU-E growth in the bone marrow from normal mice. As different sensitivities of
erythroid and myeloid progenitor cells to nitric oxide (NO) were found, we considered the possibility that the
observed effects of IL-17 were mediated by NO. The effects of recombinant mouse IL-17, NO donor (sodium
nitroprusside - SNP) and two NO synthases inhibitors (L-NAME and aminoguanidine) on erythroid progenitor
cells growth, as well as the ability of IL-17 to induce nitric oxide production in murine bone marrow cells, were
examined. In addition, we tested whether the inhibition of CFU-E colony formation by IL-17 could be corrected
by erythropoietin (Epo), the principal regulator of erythropoiesis. We demonstrated that IL-17 can stimulate low
level production of NO in murine bone marrow cells. Exogenously added NO inhibited CFU-E colony formation,
whereas both L-NAME and aminoguanidine reversed the CFU-E suppression by IL-17 in a dose-dependent
manner. The inhibition of CFU-E by IL-17 was also corrected by exposure to higher levels of Epo. The data
obtained demonstrated that at least some of the IL-17 effects in bone marrow related to the inhibition of CFU-E,
were mediated by NO generation. The fact that Epo also overcomes the inhibitory effect of IL-17 on CFU-E
suggests the need for further research on their mutual relationship and co-signalling.},
DOI = {}
}