@Article{,
AUTHOR = {Davide Agnello, Patrizia Mascagni, Riccardo Bertini, Pia Villa, Giorgio Senaldi, Pietro Ghezzi},
TITLE = {Granulocyte colony-stimulating factor decreases tumor necrosis factor production in whole blood: role of interleukin-10 and prostaglandin E<sub>2</sub>},
JOURNAL = {European Cytokine Network},
VOLUME = {15},
YEAR = {2004},
NUMBER = {4},
PAGES = {323--326},
URL = {http://www.techscience.com/ECN/v15n4/66363},
ISSN = {1952-4005},
ABSTRACT = {Previous reports have indicated that the administration of granulocyte colony-stimulating factor
(G-CSF) decreases <i>ex vivo</i> tumor necrosis factor (TNF) production in humans. In this study, we report that daily
pretreatment of mice with G-CSF for three days decreases <i>ex vivo</i> lipopolysaccharide (LPS)-induced TNF
production in whole blood. Conversely, production of interleukin-10 (IL-10) and prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) is
increased. The inhibitory effect of G-CSF pretreatment on TNF production is partially reversed by addition of an
anti-IL-10 antibody, and completely reversed by combined addition of anti-IL-10 antibody and the cyclooxyge-nase
(COX) inhibitor, ketoprofen. These results suggest that G-CSF decreases TNF production in this
experimental model by increasing production of IL-10 and PGE<sub>2</sub>, which are both known inhibitors of TNF
production.},
DOI = {}
}