@Article{,
AUTHOR = {Claudio Pérez, Fermín E. González, Violeta Pavez, Aida V. Araya, Adam Aguirre, Andrea Cruzat, Juan Contreras-Levicoy, Andrés Dotte, Octavio Aravena, Lorena Salazar, Diego Catalán, Jimena Cuenca, Arturo Ferreira, Irene Schiattino, Juan C. Aguillón},
TITLE = {The –308 polymorphism in the promoter region of the tumor necrosis factor-alpha (TNF-α) gene and <i>ex vivo</i> lipopolysaccharide-induced TNF-α expression in patients with aggressive periodontitis and/or type 1 diabetes mellitus},
JOURNAL = {European Cytokine Network},
VOLUME = {15},
YEAR = {2004},
NUMBER = {4},
PAGES = {364--370},
URL = {http://www.techscience.com/ECN/v15n4/66369},
ISSN = {1952-4005},
ABSTRACT = {Several single-nucleotide polymorphisms (SNPs) have been identified in the TNF-α gene promoter.
The transition G→A at position –308 generates the TNF-α1 (G/G) and TNF-α2 (G/A or A/A) alleles, where the
polymorphic TNF-α2 allele is associated with a high, in vitro TNF-α expression and an increased susceptibility to
diverse illnesses. Here we study the association of the –308 TNF-α SNP with the susceptibility for developing
aggressive periodontitis (AP), AP combined with type 1 diabetes mellitus (DM) and DM. We also explore the
TNF-α capability expression and the presence of the –308 polymorphism. For this purpose we recruited
27 individuals with AP (AP+ group), 27 individuals with AP combined with DM (AP+/DM+ group), and
27 individuals with DM without signs of periodontitis upon clinical examination (DM+ group). The control group
was comprised of 30 subjects. Genotyping for TNF-α promoter was performed by PCR-RFLP analysis. For TNF-α
expression we used a blood culture system.},
DOI = {}
}