@Article{,
AUTHOR = {Rafael Botella-Estrada, Marta Escudero, José E. O’Connor, Eduardo Nagore, Bernardo Fenollosa, Onofre Sanmartín, Celia Requena, Carlos Guillén},
TITLE = {Cytokine production by peripheral lymphocytes in melanoma},
JOURNAL = {European Cytokine Network},
VOLUME = {16},
YEAR = {2005},
NUMBER = {1},
PAGES = {47--55},
URL = {http://www.techscience.com/ECN/v16n1/66236},
ISSN = {1952-4005},
ABSTRACT = {Background. The differentiation of T cells towards a T helper 1 (Th1) or Th2 phenotype based on
their profile of cytokine production, is of great relevance in the regulation of immune responses. We have
determined by flow cytometry, the expression of selected Th1 and Th2 cytokines by activated T cells in whole
blood samples (WB) from normal donors and from patients with different clinical stages of melanoma in different
clinical stages. Methods. WB samples from 6 normal donors and 19 patients with melanoma were activated over
4 hours with PMA + ionomycin in presence or absence of a protein secretion inhibitor. Following surface staining
(CD3-Cy5+CD8-FITC), fixation and permeabilization, cells were stained with PE-labelled antibodies against Th1
cytokines (IL-2, IFN-γ, TNF-α) and Th2 cytokines (IL-4, IL-10). Results. The most relevant results were related
to IFN-γ and IL-10 production. The percentage of IFN-γ producer cells was significantly lower in melanoma
patients, independent of the stage, than in controls. IL-10 production was significantly increased in melanoma
patients with respect to normal donors. Conclusions. Our data support the notion that the pattern of cytokines
produced by lymphocytes from melanoma patients may help to explain the impairment in their T cell immune
response. More extensive studies regarding the pattern of cytokines, not only in peripheral blood, but also in
tumour tissue and sentinel lymph nodes, are needed to confirm these data.},
DOI = {}
}