TY  - EJOU
AU  - Papadopoulos, D. P. 
AU  - Moyssakis, I. 
AU  - Makris, T. K. 
AU  - Poulakou, M. 
AU  - Stavroulakis, G. 
AU  - Perrea, D. 
AU  - Votteas, V. E. 

TI  - Clinical significance of matrix metalloproteinases activity in acute myocardial infarction
T2  - European Cytokine Network

PY  - 2005
VL  - 16
IS  - 2
SN  - 1952-4005

AB  - Matrix metalloproteinases (MMP) degrade myocardial fibrillar collagen in acute myocardial
infarction (MI) patients. Their activity is tightly controlled in normal myocardium by a family of closely related
tissue inhibitors known as TIMP. An imbalance in their activity might contribute to post-MI remodeling. Plasma
levels of MMP-1, TIMP-1 and MMP-1/TIMP-1 complex were measured, using relevant ELISA kits, in 24 (22
males-2 females), acute MI patients with a mean age 59 ± 14 years. Blood samples were taken on admission
(0h),and 3h, 6h, 9h, 18h, 24h, 36h, 48h, 3<sup>rd</sup>, 4<sup>th</sup>, 5<sup>th</sup>, 7<sup>th</sup>, 15<sup>th</sup>, 30<sup>th</sup> days after MI. All patients underwent coronary
arteriography with ventriculography for estimation of left ventricular ejection fraction (LVEF) and extent of
coronary artery diseases, and echocardiographic study for measuring end-diastolic diameter (EDD). Ten patients
with an LVEF < 45%, an EDD > 47.5mm, and heart failure symptoms were included in group A and compared
against 12 patients with an LVEF > 45% an EDD < 47.5mm in group B.Mean plasma concentrations of MMP-1
were higher by 21% in group A (1.3 ± 0.2 ng/mL) compared to group B (1 ± 0.1 ng/mL) over the total study period.
TIMP-1 plasma concentrations showed very little difference between the 2 groups, (704 ± 213 ng/mL versus
691 ± 165 ng/mL, (6%)) Finally, plasma concentrations of MMP-1/TIMP-1 complex were lower by -36% in group
A with a mean value of 2.7 ± 0.6 ng/mL versus 3.7 ± 0.5 ng/mL in group B. Mean values for the differences were
significant at time points 0, 6, 18, 24 and 48 hours for MMP-1 (p < 0.036), and on 48h and the 4<sup>th</sup> day for
MMP-1/TIMP-1 complex (p < 0.031). Moreover, a good correlation was found between plasma concentrations of
creatine kinase (CK) and MMP-1 at 18h (r = 0.422, p = 0.041) and on the 4<sup>th</sup> day (r = 0.67, p = 0.046), and TIMP-1
on the 4<sup>th</sup> day (r = 0.67, p = 0.047). Additionally, mean values for LVEF were 35.8 ± 8.8% in group A versus
51.2 ± 1.8% (p = 0.00014) in group B. Also, the EDD in-group A was 52.1 ± 6.9 mm versus 42.9 ± 3.2 mm in group
B (p = 0.00013). In acute MI patients, increased MMP-1, with no change in TIMP-1, is associated with left
ventricular dysfunction and dilatation, suggesting that increased collagenolytic activity contributes to loss of LV
function.
KW  - matrix metalloproteinases
KW  -  acute myocardial infarction
KW  -  extracellular matrix

DO  -