@Article{,
AUTHOR = {Mahmoud Abu Elhija, Hadas Potashnik, Eitan Lunenfeld, Gad Potashnik, Stefan Schlatt, Eberhard Nieschlag, Mahmoud Huleihel},
TITLE = {Testicular interleukin-6 response to systemic inflammation},
JOURNAL = {European Cytokine Network},
VOLUME = {16},
YEAR = {2005},
NUMBER = {2},
PAGES = {167--172},
URL = {http://www.techscience.com/ECN/v16n2/66230},
ISSN = {1952-4005},
ABSTRACT = {Spermatogenesis is a highly controlled process of proliferation, meiosis, and differentiation.
Systemic infection and chronic inflammation can impair testicular steroidogenesis and spermatogenesis. In this
study, we examined the effect of systemic infection – intraperitoneal (i.p.) injection of lipopolysaccharide (LPS) –
on the expression levels of IL-6 in the testis of sexually immature and adult mice. IL-6 levels in testicular
homogenates of immature mice were significantly higher than in mature mice (both protein and RNA levels),
before and after LPS injection. Injection of LPS (i.p.) into mature mice over 3 hours, significantly increased
testicular IL-6 protein and mRNA levels (as demonstrated by ELISA and RT-PCR respectively) compared to the
control group. Injection of LPS over 24 hours significantly increased IL-6 mRNA expression, but it did not
significantly affect IL-6 protein levels in the homogenates. In contrast, stimulation of immature mice with LPS (2,
20 or 100 lg/mL) over 3 hours or LPS (2 or 20 lg/mL) over 24 hours, significantly increased testicular IL-6 (both
protein and mRNA expression). The levels of testicular IL-6 (protein) in the homogenates were not significantly
increased after stimulation with 100 lg/mL over 24 hours, but they were significantly increased at the mRNA level.
Our results demonstrate, for the first time, the over-expression of IL-6 in testicular homogenates of mature and
immature mice following systemic inflammation (i.p. injection of LPS). These results suggest the possibility of the
involvement of systemic infection/inflammation, through the elevation of testicular IL-6, in testicular functions,
which may affect male fertility. Also, high levels of IL-6 during pathological conditions, could play a role in
protecting testicular tissue.},
DOI = {}
}