@Article{,
AUTHOR = {Nicolas Cagnard, Franck Letourneur, Abdellatif Essabbani, Valérie Devauchelle, Sylvie Mistou, Audrey Rapinat, Charles Decraene, Catherine Fournier, Gilles Chiocchia},
TITLE = {Interleukin-32, CCL2, PF4F1 and GFD10 are the only cytokine/chemokine genes differentially expressed by <i>in vitro</i> cultured rheumatoid and osteoarthritis fibroblast-like synoviocytes},
JOURNAL = {European Cytokine Network},
VOLUME = {16},
YEAR = {2005},
NUMBER = {4},
PAGES = {289--292},
URL = {http://www.techscience.com/ECN/v16n4/66206},
ISSN = {1952-4005},
ABSTRACT = {Since cytokines and chemokines are important actors in rheumatoid arthritis (RA), the aim of this
study was to compare the gene expression profiles in cultured fibroblast-like synoviocytes (FLS) obtained from
patients with either RA, or osteoarthritis (OA), focusing our analysis on genes for cytokines and chemokines, and
their respective receptors. Gene expression in cultured FLS (third passage) from eight patients with RA (RA-FLS)
were compared with gene expression in cultured FLS from nine patients with OA (OA-FLS) using Affymetrix
Human Genome U133 Plus 2.0 Array microarray, allowing analysis of over 54,000 transcripts. Among the 171
genes studied (241 probes), limiting the selection of differentially expressed genes to a significant value (p < 0.05),
and a differential ratio of expression > 1.6, only four genes, namely IL-32, CCL2, PF4F1 and GDF10 were found
to be differentially expressed. Out of these four genes, only higher expression of CCL2 has been reported
previously in RA. The newly described cytokine IL-32 was the most prominently differentially expressed gene in
the present study, with higher expression in RA-FLS than in OA-FLS (p < 0.0073). IL-32 might have a previously
unidentified pivotal role in RA.},
DOI = {}
}