@Article{,
AUTHOR = {Stéphane Potteaux, Virginie Deleuze, Régine Merval, Michel F. Bureau, Bruno Esposito, Daniel Scherman, Alain Tedgui, Ziad Mallat},
TITLE = {In vivo electrotransfer of interleukin-10 cDNA prevents endothelial upregulation of activated NF-κB and adhesion molecules following an atherogenic diet},
JOURNAL = {European Cytokine Network},
VOLUME = {17},
YEAR = {2006},
NUMBER = {1},
PAGES = {13--18},
URL = {http://www.techscience.com/ECN/v17n1/66163},
ISSN = {1952-4005},
ABSTRACT = {Objectives. Interleukin (IL)-10 has anti-atherogenic properties. However, the molecular mechanisms
involved in IL-10 protection against atherosclerosis in vivo remain poorly understood. In this study, we
examined the effect of IL-10 cDNA in vivo electrotransfer on diet-induced, endothelial activation. Methods.
C57BL/6J mice were fed an atherogenic diet for 10 days. Expression of VCAM-1 and ICAM-1 was examined in
the aortic sinus, a region predisposed to atherogenesis in mice, using immunohistochemistry. NF-κB activation
was examined using a monoclonal antibody that selectively reacts with the activated form of the p65 subunit.
Results. We detected a low basal expression of activated NF-κB, VCAM-1 and ICAM-1 in the endothelium of the
aortic sinus. Endothelial expression of activated NF-κB, VCAM-1 and ICAM-1 was markedly increased after 10
days on the atherogenic diet (p < 0.001). In vivo electrotransfer of a murine IL-10-encoding plasmid completely
prevented diet-induced endothelial upregulation of activated NF-κB, VCAM-1 and ICAM-1 (p < 0.01). Conclusion.
In vivo electrotransfer of IL-10 cDNA prevents diet-induced endothelial activation. These results suggest that
the protective effects of IL-10 may already occur in the very early stages of atherogenesis.},
DOI = {}
}