TY - EJOU AU - Chennaoui, Mounir AU - Drogou, Catherine AU - Guezennec, Charles-Yannick AU - Gomez-Merino, Danielle TI - Inﬂuence of a high carbohydrate diet on the functional activity of 5-HT_1B/1D receptors on human peripheral blood lymphocytes during intense military training T2 - European Cytokine Network PY - 2006 VL - 17 IS - 1 SN - 1952-4005 AB - The present study was undertaken to examine the effect of a high carbohydrate diet on the functional activity of 5-HT_1B/1D receptors in human peripheral blood lymphocytes, and on serum cortisol and plasma cytokine responses during intense military training. Thirty two male soldiers (mean age: 21 ± 2 years) were randomly assigned to two groups and received either 3200 kcal/24 h [13440 kJ; habitual diet group (HD)] or 4200 kcal/24 h [17640 kJ, high carbohydrate diet group (HCD)] by adding 1000 kcal (4200 kJ) of fruit jelly to the HD. They took part in a three-week training program followed by a ﬁve-day combat course. Blood samples were collected from each group before entry into the commando training and after the ﬁve-day combat course. The results of [³⁵S] GTPγS binding assays showed that h5-HT_1B/1D receptors were desensitized after the training program in the HD group, whereas no change was observed between the beginning and the end of the military training in the HCD group [(HD : IC50 = 100 ± 14 nM to 544 ± 178 nM; n = 16) and (HCD: IC50 = 68 ± 14 nM to 101 ± 22 nM; n = 16)]. Serum cortisol was only signiﬁcantly increased after the commando training in the HD group (from 532.2 ± 30 to 642 ± 45 nmol·L^-1, p < 0.05), whereas values were not signiﬁcantly changed in the HCD group (441 ± 31 to 502 ± 40 nmol·L-1). No changes were observed in IL-10, TNF-α and IFN-γ levels after the training program in either group. Carbohydrate ingestion or additional dietary energy during repeated bouts of high-intensity exercise could attenuate the alterations in immune function via 5-HT_1B/1D receptors and the action of 5-HT moduline, an endogenous tetrapeptide (Leu-Ser-Ala-Leu) that speciﬁcally modulates the sensitivity of 5-HT_1B/1D receptors. KW - 5-HT_1B/1D receptors KW - T lymphocyte KW - multi-stressors KW - carbohydrate KW - training DO -