@Article{,
AUTHOR = {Carolina Figueroa, Alexis Peralta, Luisa Herrera, Pedro Castro, Andrea Gutiérrez, Jorge Valenzuela, Juan C. Aguillón, Rodrigo Quera, Marcela A. Hermoso},
TITLE = {NOD2/CARD15 and Toll-like 4 receptor gene polymorphism in Chilean patients with inflammatory bowel disease},
JOURNAL = {European Cytokine Network},
VOLUME = {17},
YEAR = {2006},
NUMBER = {2},
PAGES = {125--130},
URL = {http://www.techscience.com/ECN/v17n2/66158},
ISSN = {1952-4005},
ABSTRACT = {Crohn’s disease (CD) and ulcerative colitis (UC) are multifactorial diseases with a genetic
background. Genes related to the innate immune response have been observed to be involved. Polymorphisms of
Toll-like receptor 4 (TLR4) and CARD15/NOD2 are thought to be involved in the pathogenesis of inflammatory
bowel disease (IBD). There is no information about the frequency of these polymorphisms in South American and
Chilean populations. Aim. To investigate the distribution of CARD15/NOD2 (Arg702Trp, Gly908Arg and
Leu1007fsinsC) and TLR4 (Asp299Gly) polymorphisms in Chilean patients with IBD. Methods. DNA was obtained
from 22 CD, 22 UC patients and 20 healthy individuals. Genotyping was performed by allele-specific PCR and by
PCR-RFLP analysis. Clinical and demographic features were characterized. Results. Among the CD patients, the
clinical pattern was deemed inflammatory in 14, while five had penetrating and five stricturing, variants. One
patient had esophageal involvement, five perianal, seven ileal and in 16 the colon was involved. Among the UC
patients, two had proctitis, two proctosigmoiditis, four left-sided colitis and 14 pancolitis. NOD2/CARD15 analysis
revealed the presence of the 702Trp allele in two CD patients (both heterozygotes), 1007fsinsC in one CD patient
(heterozygote) while 908Arg was found in one UC patient. The 299Gly TLR4 allele was identified in one UC and
one CD patient. Conclusion. This genetic study shows that the alleles frequently associated with IBD (1007fsinsC,
908Arg and 702Trp in NOD2/CARD15 and 299Gly TLR4) have a low incidence in Chilean, IBD patients, which
is similar to European populations. It is possible that, in addition to environmental factors, other genetic
polymorphisms may be involved in the pathogenesis of the disease in Chilean, IBD patients.},
DOI = {}
}