TY  - EJOU
AU  - Zhou, Lei 
AU  - Li, Yixing 
AU  - Xia, Tao 
AU  - Feng, Shengqiu 
AU  - Chen, Xiaodong 
AU  - Yang, Zaiqing 

TI  - Resistin overexpression impaired glucose tolerance in hepatocytes
T2  - European Cytokine Network

PY  - 2006
VL  - 17
IS  - 3
SN  - 1952-4005

AB  - Resistin is a 12.5-kDa cysteine-rich protein secreted from adipose tissue and is an important factorlinking obesity with insulin resistance. Here, we investigated the effect of resistin on glucose tolerance in adulthuman hepatocytes (L-02 cells). In this study, resistin cDNA was transfected into L-02 cells, and glucoseconcentration and glucokinase activity were determined subsequently. The data indicated resistin impaired,insulin-stimulated glucose utilization, which implied liver was a target tissue of resistin. To understand itsmolecular mechanism, mRNA levels of key genes in glucose metabolism and insulin signaling pathway wereanalyzed. The results demonstrated resistin-stimulated expression of glucose-6-phosphatase (G6Pase), sterolregulatory element-binding protein 1c (SREBP1c) and suppressor of cytokine signaling 3 (SOCS-3), repressedexpression of peroxisome proliferator-activated receptor γ (PPARγ) as well as insulin receptor substrate 2 (IRS-2).Given that glucokinase (GK) activity and glucose transporter 2 (GLUT2) expression were not altered, wepresumed that resistin did not effect them. Moreover, resistin lowered mRNA levels of IRS-2 while stimulatingSOCS-3 expression, which suggests it impairs glucose tolerance by blocking the insulin signal transductionpathway.
KW  - glucose tolerance
KW  -  insulin resistance
KW  -  resistin
KW  -  rosiglitazone

DO  - 10.1684/ecn.2006.0039