@Article{ecn.2006.0045,
AUTHOR = {Amel Boumendjel, Lina Tawk, René de Waal Malefijt, Vera Boulay, Hans Yssel, Jérôme Pène},
TITLE = {IL-27 induces the production of IgG1 by human B cells},
JOURNAL = {European Cytokine Network},
VOLUME = {17},
YEAR = {2006},
NUMBER = {4},
PAGES = {281--289},
URL = {http://www.techscience.com/ECN/v17n4/66141},
ISSN = {1952-4005},
ABSTRACT = {It has been reported that IL-27 specifically induces the production of IgG2a by mouse B cells and
inhibits IL-4-induced IgG1 synthesis. Here, we show that human naïve cord blood expresses a functional IL-27
receptor, consisting of the TCCR and gp130 subunits, although at lower levels as compared to naïve and memory
splenic B cells. IL-27 does not induce proliferative responses and does not increase IgG1 production by
CD19+
CD27+
memory B cells. However, it induces a low, but significant production of IgG1 by naïve
CD19+
CD27–
IgD+
IgG–
spleen and cord blood B cells, activated via CD40, whereas it has no effect on the
production of the other IgG subclasses. In addition, IL-27 induces the differentiation of a population of B cells that
express high levels of CD38, in association with a down-regulation of surface IgD expression, and that are surface
IgG+/int
, CD20low, CD27high
, indicating that IL-27 promotes isotype switching and plasma cell differentiation of
naive B cells. However, as compared to the effects of IL-21 and IL-10, both switch factors for human IgG1 and
IgG3, those of IL-27 are modest and regulate exclusively the production of IgG1. Finally, although IL-27 has no
effect on IL-4 and anti-CD40-induced C∈ germline promoter activity, it up-regulates IL-4-induced IgE production
by naive B cells. These results point to a partial redundancy of switch factors regulating the production of IgG1
in humans, and furthermore indicate the existence of a common regulation of the human IgG1and murine IgG2a
isotypes by IL-27.},
DOI = {10.1684/ecn.2006.0045}
}