@Article{ecn.2007.0081,
AUTHOR = {Junsuke Shirai, Kikumi Ogihara, Ai Masumoto, Kazuki Morioka, Yuko Naya, Yoshinori Tsuchiya, Yuichi Yokomizo},
TITLE = {Continuous large-scale production of the cytokine CXCL8 from a novel porcine cell line},
JOURNAL = {European Cytokine Network},
VOLUME = {18},
YEAR = {2007},
NUMBER = {1},
PAGES = {10--17},
URL = {http://www.techscience.com/ECN/v18n1/66007},
ISSN = {1952-4005},
ABSTRACT = {Cytokine production from two unstimulated porcine cell lines (SL-24 and SK-L) was examined
using porcine cytokine detection ELISA kits and RT-PCR. Porcine IL-1α, IL-6, and CXCL8 were detected in all
samples examined. In particular, the SL-24 cell line (derived from bone marrow cells of a malignant
lymphoma-affected pig), produced large amounts of porcine CXCL8. Flow cytometer analysis showed the cell line
to be strongly CD44 positive, and was therefore considered to be of monocyte or macrophage origin. Porcine
CXCL8 production was greatest (83.86 ± 32.33 ng/mL) at six days post-cultivation. The SK-L cell line (derived
from porcine kidney) also produced CXCL8, but production was less than 1.5 ng/mL. Porcine CXCL8 from the
SL-24 cell line, induced chemotactic activity in porcine neutrophils, while the production of CXCL8 from the
SL-24 cell line was inhibited by dexamethasone, which suggests that the mechanism of CXCL8 production is
related to an NF-jB binding site. The production of CXCL8 from the SL-24 cell line was enhanced by the addition
of recombinant porcine IL-15, which is the first reported observation of such CXCL8 production. Cloning of the
SL-24 cell line by limited dilution revealed two types of cells present in the starting population. One cell type,
designated as long-form cells (LC), produced large amounts of CXCL8, while the other, designated short-form
cells (SC), produced small amounts of the cytokine. The LC cells were adapted to grow in serum-free medium in
which they produced large amounts of CXCL8. The large-scale production of porcine CXCL8 from the SF-24 cell
line will be of value in determining the mechanism of cytokine production and as a source of naturally produced
porcine CXCL8.},
DOI = {10.1684/ecn.2007.0081}
}