@Article{ecn.2007.0082,
AUTHOR = {Jacqueline Mohler, Pierre Moine, Esther Azoulay-Dupuis, Dominique Henin, Bruno Fantin},
TITLE = {Polyvalent 23 epitope polysaccharide pneumonia vaccine induced effective protection through strain-adapted effector mechanisms as demonstrated by the different cytokine responses in mice challenged with two different strains of <i>Streptococcus pneumoniae</i>},
JOURNAL = {European Cytokine Network},
VOLUME = {18},
YEAR = {2007},
NUMBER = {1},
PAGES = {23--30},
URL = {http://www.techscience.com/ECN/v18n1/66010},
ISSN = {1952-4005},
ABSTRACT = {We used a Balb/c mouse model of pneumococcal pneumonia to investigate the protection
mechanisms induced by immunization with a polyvalent 23 epitope polysaccharide pneumonia vaccine. Groups
of mice were injected x 4 times s.c. within one month, with this vaccine preparation. Mice were subsequently
challenged at day 45, with a lethal, intratracheal inoculum of two strains of <i>Streptococcus pneumoniae</i> – either a
highly virulent and strongly immunogenic serotype 3 strain (P4241), or a less virulent and weakly immunogenic
serotype 19F strain (P15986). The intratracheal <i>S. pneumoniae</i> challenge-induced lethality, antibody response,
bacterial clearance, and cytokine secretions were monitored to analyze the strain-adapted effector mechanisms.
Pulmonary levels of TNFα, IL-6, IL-1β, MIP-1α, KC, MCP-1/JE and MIP-2 cytokines were determined up to
48 hours post-infection. Survival rates were 82% and 100% among vaccinated animals challenged at day 45 with
P4241, and P1598 mice respectively, and 0% in non-vaccinated mice (p < 0.001). Survival was associated with a
rapid bacterial clearance from blood and lungs, which similar for the two strains. Immunization induced a
serotype-specific antibody response. Kinetics of the cytokine profile in the lung following intratracheal inoculation
with the 4241 strain was different in animals vaccinated 45 days previously, compared to naïve, control mice.
Generally speaking the bacterial-induced inflammatory cytokine response induced with the 4241 strain was much
weaker in vaccinated animals than in control mice. The only cytokines showing a greater increase in vaccinated
mice compare to control animals were IL-1β, KC and MCP-1. Production of TNFα and IL-6 was lower in
vaccinated animals than in controls. At variance with the previous bacteria strain-induced cytokine profile,
infection with the P15986 strain induced a strong inflammatory response, with a substantial increase in all the
cytokine tested, which was similar in vaccinated and in naïve, control animals, except for MIP-1α, which was the
only mediator significantly more produced by vaccinated animals than by naïve, control mice following P15986
infection. The distinct cytokine profiles, which were observed in this study depending upon the two strains of
<i>S. pneumoniae</i> used for challenge, demonstrated that protection against each strain was obtained through a
different defence strategy.},
DOI = {10.1684/ecn.2007.0082}
}