@Article{ecn.2007.0084,
AUTHOR = {Gy. Farkas Jr., P. Hofner, A. Balog, T. Takács, A. Szabolcs, Gy. Farkas, Yvette Mándi},
TITLE = {Relevance of transforming growth factor-β1, interleukin-8, and tumor necrosis factor-α polymorphisms in patients with chronic pancreatitis},
JOURNAL = {European Cytokine Network},
VOLUME = {18},
YEAR = {2007},
NUMBER = {1},
PAGES = {26--32},
URL = {http://www.techscience.com/ECN/v18n1/66011},
ISSN = {1952-4005},
ABSTRACT = {Cytokine regulation may be an important factor in the susceptibility for the development of chronic
pancreatitis; transforming growth factor-β1 (TGF-β1) plays a central role in the pathogenesis of pancreatic
fibrogenesis. The aim of our study was to analyse the relevance of TGF-b1, interleukin-8 (IL-8) and tumor necrosis
factor-α (TNF-α) polymorphisms in patients with chronic pancreatitis.Patients: of the 83 patients enrolled in the
study, 43 were treated medically and 40 patients underwent surgical intervention. Healthy blood donors (n = 75)
served as controls.Methods: the polymorphisms of TGF-β1 +869 T→ C and IL-8 -251 T→A were determined by
the ARMS method, while that of TNF-α -308 was investigated using NcoI RFLP.Results: there was a higher fre-quency
(50%) of the TT genotype of TGF-β1 +869, with a concomitantly higher TGF-β1 level in the plasma
(5.2 ± 1.7 ng/mL) of patients with chronic pancreatitis than in healthy blood donors (28% and 2.8 ± 0.9 ng/mL
respectively). The number of TT homozygotes differed significantly between the patients who underwent surgical
intervention and the controls, and even between the surgical and the non-surgical patients. The frequency of the
T/A genotype with higher IL-8 production, was significantly higher in both groups of patients than in the controls
(58% and 58% versus 40%). No correlation was found between the TNF-α -308 polymorphism and chronic
pancreatitis.Conclusions: correlations of the TGF-β1 and IL-8 single nucleotide polymorphisms (SNPs) with
chronic pancreatitis underline the importance of these cytokines in the pathomechanism of the disease. Moreover,
it seems that the TT genotype of +869 TGF-β1 might be a risk factor for the development of a severe form of
chronic pancreatitis, and could serve as a prognostic sign for any future surgical intervention or even repeat
surgery. Further studies on a larger group of patients, in addition to a follow-up study, are necessary to confirm
this preliminary observation.},
DOI = {10.1684/ecn.2007.0084}
}