@Article{ecn.2007.0085,
AUTHOR = {Celia Murciano, Alberto Yánez, M. Luisa Gil, Daniel Gozalbo},
TITLE = {Both viable and killed <i>Candida albicans</i> cells induce <i>in vitro</i> production of TNF-α and IFN-γ in murine cells through a TLR2-dependent signalling},
JOURNAL = {European Cytokine Network},
VOLUME = {18},
YEAR = {2007},
NUMBER = {1},
PAGES = {33--37},
URL = {http://www.techscience.com/ECN/v18n1/66013},
ISSN = {1952-4005},
ABSTRACT = {The in vitro production of TNF-α and IFN-γ in response to <i>Candida albicans</i> was investigated in
wild-type, TLR2-/- and TLR4-/- murine cells. TLR2-/- resident peritoneal macrophages showed a strong
impairment of TNF-α production in response to viable and non-viable (heat-killed, antimycotic-treated and
formaldehyde-fixed) yeasts and hyphae (germ tube-bearing cells) of the high virulence <i>C. albicans</i> ATCC 26555
strain, as compared with macrophages from wild-type and TLR4-/- mice. The in vitro production of IFN-γ was
investigated in murine splenocytes obtained three days after intravenous injection with the low virulence,
non-germinative <i>C. albicans</i> PCA2 strain, and again, TLR2-/- splenocytes showed a strong impairment of the in
vitro production of IFN-γ in response to non-viable (heat-killed, antimycotic-treated and formaldehyde-fixed)
<i>C. albicans</i> ATCC 26555 yeasts, as compared with splenocytes of TLR4-/- and wild type mice. These results
indicate that the TLR2-mediated recognition of <i>C. albicans</i> leading to a proinflammatory Th1 host response
appears to be well conserved in killed <i>C. albicans</i> cells, regardless of the inactivating treatment employed.},
DOI = {10.1684/ecn.2007.0085}
}