@Article{ecn.2007.0088,
AUTHOR = {Qingxia Yao, Ping Qian, Yi Cao, Yannan He, Youhui Si, Zhuofei Xu, Huanchun Chen},
TITLE = {Synergistic inhibition of pseudorabies virus replication by porcine alpha/beta interferon and gamma interferon <i>in vitro</i>},
JOURNAL = {European Cytokine Network},
VOLUME = {18},
YEAR = {2007},
NUMBER = {2},
PAGES = {71--77},
URL = {http://www.techscience.com/ECN/v18n2/65998},
ISSN = {1952-4005},
ABSTRACT = {Interferon (IFN) is crucial for initiating the innate immune response and for the generation of the
adaptive response. IFN, in most species, comprises IFN-alpha (IFN-α), IFN-beta (IFN-β) and IFN-gamma (IFN-γ).
In this study, we compared the capacity of porcine IFN-α, -β and -γ, or a combination of them, to protect IBRS-2
cells (porcine kidney cells) from infection with pseudorabies virus (PRV). The results demonstrated that porcine
IFN-β (PoIFN-β) was the most efficient of the three IFNs in conferring resistance PRV infection; 100 U/mL
PoIFN-β inhibited PRV plaque formation 5.3-fold. Compared with PoIFN-β, porcine IFN-c (PoIFN-γ) was less
capable of inhibiting PRV plaque formation (3.3-fold inhibition). Porcine IFN-α (PoIFN-α) had the least capability
of the three PoIFNs, and inhibited PRV plaque formation only 1.26-fold. The inhibitory capacity increased to only
2.3-fold with a treatment of 12,800 U/mL PoIFN-α. A combination of PoIFN-γ and PoIFN-α or PoIFN-β inhibited
PRV plaque formation 12.8-fold or 100-fold, respectively. Treatment of IBRS-2 cells with PoIFN-α/β and PoIFN-γ
inhibited PRV replication 29- or 146-fold. Additionally, real-time PCR analyses of the PRV immediate early (IE)
gene revealed that IE mRNA expression was profoundly decreased in cells stimulated with PoIFN-α/β and
PoIFN-γ (23.8–133.0-fold) compared with vehicle-treated cells. All the findings indicate that PoIFN-γ acts
synergistically with other PoIFNs (PoIFN-α and -β) to potently inhibit PRV replication in vitro.},
DOI = {10.1684/ecn.2007.0088}
}