TY - EJOU AU - Yao, Qingxia AU - Qian, Ping AU - Cao, Yi AU - He, Yannan AU - Si, Youhui AU - Xu, Zhuofei AU - Chen, Huanchun TI - Synergistic inhibition of pseudorabies virus replication by porcine alpha/beta interferon and gamma interferon in vitro T2 - European Cytokine Network PY - 2007 VL - 18 IS - 2 SN - 1952-4005 AB - Interferon (IFN) is crucial for initiating the innate immune response and for the generation of the adaptive response. IFN, in most species, comprises IFN-alpha (IFN-α), IFN-beta (IFN-β) and IFN-gamma (IFN-γ). In this study, we compared the capacity of porcine IFN-α, -β and -γ, or a combination of them, to protect IBRS-2 cells (porcine kidney cells) from infection with pseudorabies virus (PRV). The results demonstrated that porcine IFN-β (PoIFN-β) was the most efficient of the three IFNs in conferring resistance PRV infection; 100 U/mL PoIFN-β inhibited PRV plaque formation 5.3-fold. Compared with PoIFN-β, porcine IFN-c (PoIFN-γ) was less capable of inhibiting PRV plaque formation (3.3-fold inhibition). Porcine IFN-α (PoIFN-α) had the least capability of the three PoIFNs, and inhibited PRV plaque formation only 1.26-fold. The inhibitory capacity increased to only 2.3-fold with a treatment of 12,800 U/mL PoIFN-α. A combination of PoIFN-γ and PoIFN-α or PoIFN-β inhibited PRV plaque formation 12.8-fold or 100-fold, respectively. Treatment of IBRS-2 cells with PoIFN-α/β and PoIFN-γ inhibited PRV replication 29- or 146-fold. Additionally, real-time PCR analyses of the PRV immediate early (IE) gene revealed that IE mRNA expression was profoundly decreased in cells stimulated with PoIFN-α/β and PoIFN-γ (23.8–133.0-fold) compared with vehicle-treated cells. All the findings indicate that PoIFN-γ acts synergistically with other PoIFNs (PoIFN-α and -β) to potently inhibit PRV replication in vitro. KW - pseudorabies virus KW - porcine interferon-alpha KW - porcine interferon-beta KW - porcine interferon-gamma KW - immediate-early gene DO - 10.1684/ecn.2007.0088