@Article{ecn.2007.0091,
AUTHOR = {Denis Arsenijevic, Noemi Hernadfalvi, Claudia von Meyenburg, Brigitte Onteniente, Denis Richard, Wolfgang Langhans},
TITLE = {Role for nerve growth factor in the <i>in vivo</i> regulation of glutathione in response to LPS in mice},
JOURNAL = {European Cytokine Network},
VOLUME = {18},
YEAR = {2007},
NUMBER = {2},
PAGES = {93--101},
URL = {http://www.techscience.com/ECN/v18n2/66001},
ISSN = {1952-4005},
ABSTRACT = {Since the redox state regulator glutathione (GSH), which influences lipopolysaccharide (LPS)
anorexia, may be controlled by cytokines, we studied the roles of tumour necrosis factor-alpha (TNFα) and nerve
growth factor (NGF) in the GSH response to intraperitoneal (ip) LPS injection in mice. Basal NGF and total
reduced GSH (trGSH) levels were up-regulated in brain and liver of TNFα-knock-out (KO) mice, and this was
associated with attenuated LPS anorexia. The increases in NGF and trGSH presumably contributed to the
attenuated anorexia in response to LPS because transgenic mice over-expressing NGF (NGF-tg mice) also had
increased trGSH levels and displayed attenuated anorexia compared to the corresponding wild type (WT) mice.
Attenuated LPS anorexia in NGF-tg mice was accompanied by reduced serum TNFα and IFNγ levels compared
to WT mice. In response to a second injection of LPS, NGF and trGSH levels, but not TNFα levels changed. This
suggests that in vivo tissue trGSH changes following LPS in LPS-naïve or LPS-pretreated mice are regulated by
NGF rather than TNFα. The finding that genetic TNFα deficiency did not inhibit the acute trGSH response to LPS
supports this interpretation. In sum, the results indicate i) that a decrease or increase in NGF is accompanied by
a decrease or increase in trGSH levels and ii) that elevated NGF and/or trGSH levels attenuate some of the
responses to LPS such as anorexia and cytokine production.},
DOI = {10.1684/ecn.2007.0091}
}