@Article{ecn.2007.0093,
AUTHOR = {Pierre Palmer, Michael G. Tovey, Franck Raschilas, Lilia Brassart, Jean-François Meritet, Raphaël Porcher, Pierre Lebon},
TITLE = {Type I interferon subtypes produced by human peripheral mononuclear cells from one normal donor stimulated by viral and non-viral inducing factors},
JOURNAL = {European Cytokine Network},
VOLUME = {18},
YEAR = {2007},
NUMBER = {2},
PAGES = {108--114},
URL = {http://www.techscience.com/ECN/v18n2/66003},
ISSN = {1952-4005},
ABSTRACT = {Through the activation of Toll-like receptors (TLRs) or cytosolic RNA helicases, a large number of
pathogenic or synthetic components can induce the transcription of genes coding for type I interferons (IFNs).
This family of related cytokines includes notably, a single IFN-β protein and 13 different IFN-α subtypes, whose
biological activities are probably not the same. The aim of this study was to characterize the type I IFN subtypes
produced in vitro by human peripheral blood mononuclear cells (PBMCs) in response to specific inducers. Thus,
PBMCs obtained from a single donor, were exposed to various agents including Sendai virus, <i>Herpes simplex</i>
virus-1 (HSV-1), poliovirus-IgG complexes and serum from a patient with systemic lupus erythematosus (SLE).
Six hours later, mRNA was extracted and amplified by RT-PCR using primers which recognize IFN-B mRNA and
the different IFN-A mRNA subtypes. IFN-A subtypes were identified by cloning and sequencing the amplification
product. Antiviral activity was assayed in supernatant at 18 hours. Human PBMCs were found to express
constitutively type I IFNs mRNA. Antiviral activity and expression of IFN-A and IFN-B mRNA increased with
each inducing agent. Although almost all the IFN-A subtypes were detected, their relative abundance appeared to
be dependent upon the inducing agent. Incubation of PBMCs with a neutralizing monoclonal antibody directed
against the type I IFN receptor (IFNAR) did not affect the level of antiviral activity in the supernatant of induced
PBMCs. Our results suggest that the level of IFN-a expressed by PBMCs cells is independent of IFNAR feedback
signalling and that the nature of the inducing agent modifies the pattern of IFN-A subtypes preferentially
expressed by these cells.},
DOI = {10.1684/ecn.2007.0093}
}