@Article{ecn.2007.0096,
AUTHOR = {Jesus F. Bermejo-Martin, Maria C. Garcia-Arevalo, Raul Ortiz De Lejarazu, Julio Ardura, Jose M. Eiros, Ana Alonso, Vanesa Matías, Maria Pino, David Bernardo, Eduardo Arranz, Alfredo Blanco-Quiros},
TITLE = {Predominance of Th2 cytokines, CXC chemokines and innate immunity mediators at the mucosal level during severe respiratory syncytial virus infection in children},
JOURNAL = {European Cytokine Network},
VOLUME = {18},
YEAR = {2007},
NUMBER = {3},
PAGES = {45--50},
URL = {http://www.techscience.com/ECN/v18n3/65980},
ISSN = {1952-4005},
ABSTRACT = {Profiling of immune mediators in both nasal and plasma samples is a common approach to the
study of pathogenesis in respiratory viral infections. Nevertheless, mucosal immunity functions essentially
independently from peripheral immunity. In our study, 27 immune mediators were profiled in parallel, in
nasopharyngeal aspirates (NPAs) and plasma from 22 < 2 year-old children with a severe respiratory syncytial
virus infection involving the lower respiratory tract, using a multiplex assay. NPAs from 22 children with innocent
heart murmurs were used as controls. Differences in mediator concentrations between NPAs from patients and
controls were assessed using the Mann-Whitney test. Ratios between innate/adaptive-immunity mediators,
Th2/Th1-cytokines and CXC/CC-chemokines and between NPAs and plasmas were calculated and differences
were assessed using the Wilcoxon test. Associations between mediators, severity and leukocyte counts were studied
using the Spearman-Karber test. Results: increased levels of Th1 cytokines (IL-1β, IL-2, IL-12p70, IFNγ, TNFα),
Th2 cytokines (IL-13, IL-4, IL-6, IL-10), chemokines (IP-10, IL-8, MIP1a, MIP-1β), growth factors (FGFb,
PDGFbb, GCSF) and IL-1RA, IL-17 were observed in patient NPAs in comparison to controls. In the relative
comparisons between patient NPAs and plasmas, a predominance of innate immunity mediators, Th2 cytokines
and CXC chemokines was found at the mucosal level. No association between the level of each mediator in NPAs
and plasma was found. In plasma, PDGFbb, VEGF, MIP-1α, IL-8 correlated with severity; RANTES and IL-6
correlated with leukocyte counts. Conclusions: acute respiratory syncytial virus infection induces a relative
predominance of innate-immunity mediators, Th2 cytokines and CXC chemokines in the mucosal compartment
in infected children.},
DOI = {10.1684/ecn.2007.0096}
}