@Article{ecn.2007.0100,
AUTHOR = {Nívea Maria de Freitas, Ana Vitória Imbronito, Adriana Costa Neves, Fábio Daumas Nunes, Francisco Emilio Pustiglioni, Roberto Fraga Moreira Lotufo},
TITLE = {Analysis of IL-1A(-889) and TNFA(-308) gene polymorphism in Brazilian patients with generalized aggressive periodontitis},
JOURNAL = {European Cytokine Network},
VOLUME = {18},
YEAR = {2007},
NUMBER = {3},
PAGES = {142--147},
URL = {http://www.techscience.com/ECN/v18n3/65984},
ISSN = {1952-4005},
ABSTRACT = {Generalized aggressive periodontitis (GAP) comprises a group of periodontal diseases character-ized
by the rapid destruction of periodontal tissues which affect young individuals who generally present no
systemic disorders. Polymorphisms in the interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) genes have
been associated with an increased severity of chronic periodontitis. The objective of the present study was to
evaluate the association between IL-1A (-889) and TNFA (-308) gene polymorphisms and GAP. One hundred
nonsmoking subjects were selected, including 30 with GAP and 70 without periodontal disease. Gene polymor-phisms
were analyzed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)
technique. For IL-1 (-889), the frequency of genotype 1/1 was 54.3% in the control group and 56.7% in the study
group. The frequency of genotype 1/2 was 37.1% in the control group and 40% in the study group. Genotype 2/2
was detected at a frequency of 8.6% and 3.3% in the control and study groups, respectively. For TNFA, genotype
1/1 was present in 68.6% of control subjects and in 80.0% of patients with GAP, while the frequency of genotype
1/2 was 27.1% in the control group and 20% in the study group. Genotype 2/2 was present in 4.3% of control
subjects and was not detected in the study group. The frequencies of allele 1 and allele 2 of the IL-1A (-889) gene
were 72.9% and 27.1%, respectively, in the control group and 76.7% and 23.3% in the GAP group. For the TNFA
(-308) gene, the frequency of allele 1 was 82.15% in the control group and 90% in the study group, whereas the
frequency of allele 2 was 17.85% in the control group and 10% in the study group. Statistical analysis revealed
no significant difference in allele distribution for either gene between the two groups. No association was observed
between GAP and IL-1A (-889) and TNFA (-308) gene polymorphisms in Brazilian patients.},
DOI = {10.1684/ecn.2007.0100}
}