@Article{ecn.2008.0113,
AUTHOR = {Diane Agay, Maud Andriollo-Sanchez, Richard Claeyssen, Laurence Touvard, Josiane Denis, Anne-Marie Roussel, Yves Chancerelle},
TITLE = {Interleukin-6, TNF-alpha and interleukin-1 beta levels in blood and tissue in severely burned rats},
JOURNAL = {European Cytokine Network},
VOLUME = {19},
YEAR = {2008},
NUMBER = {1},
PAGES = {1--7},
URL = {http://www.techscience.com/ECN/v19n1/65941},
ISSN = {1952-4005},
ABSTRACT = {Previous studies have demonstrated the early appearance of inflammatory cytokines in the systemic
circulation after thermal injury both in humans and animals. The aim of this study was to evaluate the time course
of several cytokines, IL-6, TNF-α and IL-1β in serum, lung, liver and brain of severely burned rats during the first
week after thermal injury. Cytokine measurements were performed by enzyme-linked immunosorbent assay
(ELISA). The comparison between the sham-burned animals and animals with third-degree burns on 20% or
40% of their total body surface area allowed for the study of the inflammatory process relative to the size of the
injury. Serum IL-6 levels, which were undetectable in sham-treated animals, peaked during the first hours after
injury and were proportionate to the size of the area burned. After a few days, IL-6 increased once more, but only
in the most severely burned rats. In lung, liver and brain, low but measurable basal levels of TNF-α and IL-1 were
detected in sham-burned animals. Strikingly, IL-1β levels remained significantly elevated in the lung after injury
in animals having 20% and 40% burned skin area. Unexpectedly, both TNF-α and IL-1β production decreased
gradually in liver and brain after burn injury. Also, the inflammatory response after a burn injury appeared to
be biphasic. The first period corresponded to the early release of IL-6 into the circulation, proportional to the
severity of the injury. After a few days, a second period was marked by the extension of the inflammatory
processes from the injured area to the rest of the body, particularly to lung, which could be considered as at
potential risk of involvement in severely burned patients.},
DOI = {10.1684/ecn.2008.0113}
}