@Article{ecn.2008.0130,
AUTHOR = {Theodora Stroubini, Despoina Perrea, Apostolos Perelas, Charis Liapi, Ismini Dontas, Maria Trapali, Nikolaos Katsilambros, Panagiota Galanopoulou},
TITLE = {Effect of sibutramine on regional fat pads and leptin levels in rats fed with three isocaloric diets},
JOURNAL = {European Cytokine Network},
VOLUME = {19},
YEAR = {2008},
NUMBER = {3},
PAGES = {149--155},
URL = {http://www.techscience.com/ECN/v19n3/65934},
ISSN = {1952-4005},
ABSTRACT = {Aim. The aim of the study was to investigate: a) the differential effect of the three main macronu-trients
on food intake, fat depots and serum leptin levels and b) the impact of sibutramine on the above parameters
in rats fed <i>ad libitum </i>with three isocaloric diets. Methods. Three groups of male Wistar rats (n = 63) were fed with
a high fat diet (HFD), a high carbohydrate diet (HCD) or a high protein diet (HPD) for 13 weeks. In the last three
weeks, each group was divided into three subgroups and received sibutramine (S) either at 5 mg/kg or 10 mg/kg,
or vehicle. Food intake was measured daily during the last week of the experiment; perirenal and epididymal fat
and fat/lean ratio were calculated and serum leptin was assayed. Results. HFD-fed rats demonstrated elevated
food intake and higher regional fat depots. S at 10 mg/kg decreased food intake in the HFD and epididymal fat
in the HCD group. S also reduced perirenal fat in the HCD and HPD groups. Leptin levels were higher in rats
fed with either the HFD or the HPD compared to those fed with the HCD. Moreover, S at 10 mg/kg decreased
serum leptin levels in the HPD group. Conclusions. Results suggest a preferential effect of S on perirenal visceral
fat and support the view that body fat loss is greater when its administration is accompanied by a HCD diet. No
effect of S on leptin levels was found, besides that expected as a result of the decrease in body fat.},
DOI = {10.1684/ecn.2008.0130}
}