@Article{ecn.2008.0138,
AUTHOR = {Justin Monnier, Véronique Quillien, Claire Piquet-Pellorce, Claudine Leberre, Laurence Preisser, Hugues Gascan, Michel Samson},
TITLE = {Prokineticin 1 induces CCL4, CXCL1 and CXCL8 in human monocytes but not in macrophages and dendritic cells},
JOURNAL = {European Cytokine Network},
VOLUME = {19},
YEAR = {2008},
NUMBER = {4},
PAGES = {166--175},
URL = {http://www.techscience.com/ECN/v19n4/65923},
ISSN = {1952-4005},
ABSTRACT = {Prokineticin 1 and 2 (PROK1 and PROK2) are two small proteins largely expressed in inflamma-tory
tissues and involved in monocyte activation and differentiation. The focus of this study was to evaluate
whether PROK1 was able to induce chemokine secretion in human monocytes, in monocyte-derived macro-phages
and in monocyte-derived dendritic cells, an aspect not addressed thus far. Here, we show for the first
time, using flow cytometry, that PROK receptors 1 and 2 are present on the surface of human monocytes. Sub-sequently,
monocytes were selected to investigate the chemokine response after stimulation by PROK1. Our
results show that only three chemokines (CCL4, CXCL1 and CXCL8) were significantly induced at both the
transcript and protein level, and that PROK1 induces most potently CXCL8, in a dose-dependent manner.
From a mechanistic point of view, by blocking independently Gαi protein or intracellular calcium, monocytes
lose the ability to secrete CXCL8 in response to PROK1. Finally, we observed that CCL4, CXCL1 and CXCL8
secretion, following PROK1 induction, is only observed in monocytes and not in monocyte-derived macrophages
and dendritic cells. Our results demonstrate that, in vitro, the differentiation status of monocytes influences che-mokine
production after stimulation by PROK1, and that this chemokine production is geared toward a pro-inflammatory
response. This could represent a novel amplification loop of leukocyte recruitment, extravasation
and tissue invasion.},
DOI = {10.1684/ecn.2008.0138}
}