@Article{ecn.2008.0139,
AUTHOR = {Burcak Karaca, Yuksel Kucukzeybek, Gurbuz Gorumlu, Cigdem Erten, Mustafa K. Gul, Ercument Cengiz, Harika Atmaca, Selim Uzunoglu, Ulus A. Sanli, Bulent Karabulut, Ruchan Uslu},
TITLE = {Profiling of angiogenic cytokines produced by hormone- and drug-refractory prostate cancer cell lines, PC-3 and DU-145 before and after treatment with gossypol},
JOURNAL = {European Cytokine Network},
VOLUME = {19},
YEAR = {2008},
NUMBER = {4},
PAGES = {176--184},
URL = {http://www.techscience.com/ECN/v19n4/65924},
ISSN = {1952-4005},
ABSTRACT = {In this study, we aimed to investigate the angiogenic cytokine profiles of hormone- and drug-refractory
prostate carcinoma cell lines, PC-3 and DU-145. We also studied the effect of gossypol, a natural
polyphenolic cotton-seed extract, on the angiogenic cytokine profile of these cell lines. XTT cell proliferation
assay was used for the assessment of cytotoxicity. For apoptosis, both histone-DNA fragmentation by ELISA
assay and caspase 3/7 activity measurement were used. Angiogenic cytokine profiles of supernatants from both
cell lines, before and after treatment with gossypol, were investigated using the human angiogenesis antibody
array I<sup>®</sup>. It was shown that the two different hormone- and drug-resistant prostate cancer cell lines, PC-3 and
DU-145, constitutively express some important angiogenic cytokines, which are known to regulate tumorigenic-ity
and angiogenesis in hormone-refractory prostate cancer. However, PC-3 and DU-145 cells have distinct
angiogenic cytokine profiles. In addition, these two cells lines respond differently to gossypol treatment in terms
of cytotoxicity and angiogenic cytokine secretion. After treatment with 10 μM of gossypol, there was a 1.5-fold
decrease in angiogenin and IL-8 levels and a 1.7- and 1.8-fold decrease in ENA-78 and GRO-α levels respec-tively,
in DU-145 cells. For PC-3 cells, there were 1.6- and 1.8-fold decreases in IL-8 and VEGF levels, respec-tively.
We conclude that PC-3 and DU-145 cells secrete significant amounts of different angiogenic cytokines
that may explain their aggressive nature and metastatic potential. Gossypol treatment affects angiogenic cyto-kine
secretion from these two cell lines in a different manner. By expanding our knowledge of the heterogeneous
biological behavior of these two cell lines, novel treatment approaches can be developed for the treatment of
prostate cancer.},
DOI = {10.1684/ecn.2008.0139}
}