@Article{ecn.2009.0159,
AUTHOR = {Harika Atmaca, Gurbuz Gorumlu, Burcak Karaca, Mustafa Degirmenci, Didem Tunali, Yalcin Cirak, Duygu Unuvar Purcu, Selim Uzunoglu, Bulent Karabulut, Ulus A. Sanli, Ruchan Uslu},
TITLE = {Combined gossypol and zoledronic acid treatment results in synergistic induction of cell death and regulates angiogenic molecules in ovarian cancer cells},
JOURNAL = {European Cytokine Network},
VOLUME = {20},
YEAR = {2009},
NUMBER = {3},
PAGES = {121--130},
URL = {http://www.techscience.com/ECN/v20n3/65897},
ISSN = {1952-4005},
ABSTRACT = {In the present study, we aimed to evaluate the possible synergistic, cytotoxic effects of combination
treatment of gossypol and zoledronic acid, in human ovarian cancer cell lines, OVCAR-3 and MDAH-2774, and
to elucidate the role of this novel combination treatment on angiogenesis-related molecules in ovarian cancer.
The XTT cell viability assay was used for showing cytotoxicity. Both DNA fragmentation by ELISA assay and
caspase 3/7 activity measurement were used for demonstrating apoptosis. To elucidate the angiogenic molecules
affected by combination treatment, mRNA levels of angiogenic molecules were measured using the Human
Angiogenesis RT<sup>2</sup> Profiler<sup>TM</sup> PCR Array (SuperArray, Frederick, MD) in ovarian cancer cell lines, OVCAR-3
and MDAH-2774.The combined treatment resulted in significant, synergistic cytotoxicity, and induced apopto-sis.
This effect was observed to happen in a dose- and time-dependent manner. Moreover, the combination treat-ment
of 10 μM gossypol and 5 μM zoledronic acid resulted in significant down-regulation (≥ thee-fold) in
mRNA levels of some pivotal angiogenic molecules in OVCAR-3 and MDAH-2774 cells as compared to the
untreated control. However, this effect was different in the two ovarian cancer cell lines observed. Gossypol, in
combination with zoledronic acid, may provide a rational treatment option for ovarian cancer, not only by
direct inhibition of cell proliferation, but also inhibition of angiogenesis-related molecules.},
DOI = {10.1684/ecn.2009.0159}
}