@Article{ecn.2010.0194,
AUTHOR = {Sonia Ben-Hadj-Khalifa, Lakhdar Ghazouani, Nesrine Abboud, Ali Ben-Khalfallah, Fatma Annabi, Faouzi Addad, Wassim Y. Almawi, Touhami Mahjoub},
TITLE = {Functional interleukin-10 promoter variants in coronary artery disease patients in Tunisia},
JOURNAL = {European Cytokine Network},
VOLUME = {21},
YEAR = {2010},
NUMBER = {2},
PAGES = {136--141},
URL = {http://www.techscience.com/ECN/v21n2/65872},
ISSN = {1952-4005},
ABSTRACT = {Objectives. The contribution of interleukin (IL)-10 promoter variants -1082G/A, -819C/T, and
-592C/A to the risk of coronary artery disease (CAD) was investigated in 291 CAD patients and 291 age- and
gender-matched control subjects. Methods and results. IL-10 genotyping was performed using PCR-allele-specific
amplification (PCR-ASA). Regression analysis was employed in assessing the contribution of the
IL-10 variants to the overall CAD risk. A higher frequency of the -592A allele (p = 0.004), but not the -1082A
(p = 0.828) or -819T (p = 0.952) alleles, was seen in CAD patients. A higher frequency of -592C/A (p = 0.011),
and a lower frequency of -592C/C (p = 0.015) genotypes was noted in patients compared to healthy controls.
Regression analysis demonstrated an association of -592C/A [OR (95% CI) = 1.82 (1.02-3.23)] and -592A/A
[OR (95% CI) = 3.33 (1.27-9.09)] genotypes with 1-artery disease. Haplotype analysis revealed that none of the
eight possible IL-10 haplotypes was associated with CAD or with the severity of CAD, and was confirmed by
multivariate regression analysis, after adjusting for a number of confounders (smoking, systolic and diastolic
blood pressure, hypertension, diabetes, glucose, cholesterol, and triglycerides). Conclusions. Our results suggest
that the -592C/A, more so than the -1082G/A or the -819C/T IL-10 promoter variant alleles, may be considered
to be a risk factor for CAD in Tunisians.},
DOI = {10.1684/ecn.2010.0194}
}