@Article{ecn.2011.0275,
AUTHOR = {Jiro Hirota, Shinya Shimizu, Atsushi Watanabe, Fumiko Suzuta, Kazue Yajima, Kumiko Kimura, Makoto Haritani, Shigeki Inumaru, Yukio Yagi},
TITLE = {Establishment of a quantitative bovine CXCL8 sandwich ELISA with newly developed monoclonal antibodies},
JOURNAL = {European Cytokine Network},
VOLUME = {22},
YEAR = {2011},
NUMBER = {1},
PAGES = {73--80},
URL = {http://www.techscience.com/ECN/v22n1/65838},
ISSN = {1952-4005},
ABSTRACT = {Three IgG class anti-bovine CXCL8 (bCXCL8) monoclonal antibody (mAb)-secreting hybridomas,
SH8-8D7, SH8-12A5 and SH8-2A1, were developed. SH8-8D7 was IgG2a, and SH8-12A5 and SH8-2A1 were IgG1.
All three mAbs detected recombinant bCXCL8 (rbCXCL8) by immunoprecipitation and Western blotting. SH8-
2A1 could neutralise the chemotactic activity of rbCXCL8 towards neutrophils. The quantitative bCXCL8 ELISA
was constituted by the combination of SH8-12A5 and biotin-SH8-2A1. The detection range was 20-1000 pg/mL. A
sandwich ELISA was used to measure native bCXCL8 derived from the supernatant of cultured bovine peripheral
blood mononuclear cells stimulated with ConA, LPS or PHA. Furthermore, SH8-2A1 could detect bCXCL8 in
formalin-fixed, paraffin-embedded, pneumonic calf tissues. These findings indicate that the newly developed antibCXCL8
mAbs could contribute to research on bovine inflammatory responses and immunology.},
DOI = {10.1684/ecn.2011.0275}
}