@Article{ecn.2011.0285,
AUTHOR = {Arsene Mekinian, Ryad Tamouza, Stephan Pavy, Nicolas Gestermann, Marc Ittah, Xavier Mariette, Corinne Miceli-Richard},
TITLE = {Functional study of TNF-α promoter polymorphisms: literature review and meta-analysis},
JOURNAL = {European Cytokine Network},
VOLUME = {22},
YEAR = {2011},
NUMBER = {2},
PAGES = {88--102},
URL = {http://www.techscience.com/ECN/v22n2/65822},
ISSN = {1952-4005},
ABSTRACT = {The functional consequences of TNF-α promoter SNPs are still controversial and, to date, the functional
consequences of TNF-α haplotype combinations in healthy subjects have not been assessed. In order to assess
functional consequences of each TNF-α polymorphism and of their haplotype combination, TNF-α expression
and secretion by LPS-stimulated monocytes from 50 healthy subjects were assessed. Monocytes were isolated and
cultured for four hours, after 100 ng/mL LPS stimulation. mRNA expression was quantified using the real-time
polymerase chain reaction, and TNF-α levels were measured by enzyme-linked immunosorbent assay. Each subject
was genotyped for TNF-α -857 C/T, -238 G/A, -308 G/A polymorphisms. In order to confirm definitively the
functional consequences of these TNF-α polymorphisms, we then performed a systematic review of the literature
for TNF-α SNPs, and then a meta-analysis of the functional studies of the TNF-α -308 G/A SNP. No association
between TNF-α mRNA or protein level expression, and TNF-α -238G/A, -308G/A, -857C/T polymorphisms, studied
either independently or in haplotype combinations, was revealed. Using a meta-analysis for the TNF-α -308 G/A
polymorphism, we confirmed the absence of any association between TNF-α mRNA and protein levels, and TNF-α -
308 G/A genotypes. This study and meta-analysis of the literature confirmed the absence of any functional consequences
of the TNF-α -308G/A promoter polymorphism, either alone, or in various haplotype combinations in
healthy subjects.},
DOI = {10.1684/ecn.2011.0285}
}