@Article{ecn.2011.0292,
AUTHOR = {Cane Tulic, Miodrag Lazic, Emina Savic, Dusan Popadic, Jelena Djukic, Dusan Spasic, Milos Markovic, Zorica Ramic, Marija Mostarica-Stojkovic, Vladimir Trajkovic},
TITLE = {The preoperative activity of Th1 and Th17 cytokine axes in prediction of sepsis after radical cystectomy},
JOURNAL = {European Cytokine Network},
VOLUME = {22},
YEAR = {2011},
NUMBER = {4},
PAGES = {169--174},
URL = {http://www.techscience.com/ECN/v22n4/65814},
ISSN = {1952-4005},
ABSTRACT = {The aim of the study was to correlate the preoperative activity of Th1 and Th17 cytokine axes with the
development of sepsis after radical cystectomy. The study involved twenty patients with the infiltrative transitional
cell carcinoma of the urinary bladder without previous radiotherapy/chemotherapy, who underwent open radical
cystectomy with urinary diversion. Preoperative plasma concentrations of Th1 cytokines interleukin 12 (IL-12)
and interferon gamma (IFN-γ), and Th17 cytokines IL-23 and IL-17, were measured using ELISA. Preoperative
expression of mRNA for IL-12p35, IFN-γ, IL-23p19 and IL-17 was quantified by real-time RT-PCR using mRNA
extracted from peripheral blood mononuclear cells. Eight patients developed postoperative sepsis, diagnosed within
two weeks post-operation as systemic inflammatory response syndrome in the presence of local or systemic infection.
The preoperative basal plasma concentrations of Th1 and Th17 cytokines were slightly above the detection limits,
with a tendency toward lower concentrations in patients who developed sepsis, but the difference was not significant
(p>0.05). The preoperative expression of mRNA encoding IL-12p35 and IL-17 was significantly lower in patients
who developed sepsis (p=0.003 and p=0.028, respectively). The similar trend was observed for IL-23p19 and IFN-
γ, but the differences did not reach the statistical significance (p=0.051 and p=0.172, respectively). These data
suggest that determination of preoperative Th1 and Th17 cytokine mRNA levels might be useful in predicting
sepsis development after radical cystectomy.},
DOI = {10.1684/ecn.2011.0292}
}