@Article{ecn.2012.0298,
AUTHOR = {Lujun Zhao, Luhua Wang, Wei Ji, Mingfang Lei, Weizhi Yang, Feng-Ming Kong},
TITLE = {The influence of the blood handling process on the measurement of circulating TGF-β1},
JOURNAL = {European Cytokine Network},
VOLUME = {23},
YEAR = {2012},
NUMBER = {1},
PAGES = {1--6},
URL = {http://www.techscience.com/ECN/v23n1/65755},
ISSN = {1952-4005},
ABSTRACT = {In order to evaluate the impact of blood sample handling processes on circulating TGF-β1 levels, blood
specimens were obtained from 13 healthy volunteers using different handling processes (kept at room temperature
(RT) or on ice before centrifugation, using different centrifugal forces). TGF-β1 levels were measured using an
enzyme-linked immunosorbent assay. A paired-T test was used for statistical analysis. The TGF-β1 level in on-ice
serum was significantly lower than that in room-temperature serum (P<0.001), and both were significantly higher
than that found in on-ice plasma (P<0.001). Compared with on-ice plasma samples, the longer the samples were
kept at RT, the higher the levels of TGF-β1 in plasma (P=0.268, 0.040, and 0.0015 for 5 min, 30 min, and 60 min in
RT, respectively). Compared with plasma centrifuged at 2,500×g for 30 min, the TGF-β1 levels were much lower
than those found in plasma centrifuged at 1,200×g for 10 min (P=0.003); and a double centrifugation before TGF-β1
detection, significantly decreased the level (P<0.001). It is suggested that the optimal sampling conditions for the
detection of TGF-β1 should be plasma prepared on ice and spun down at a higher centrifugal force.},
DOI = {10.1684/ecn.2012.0298}
}