@Article{ecn.2012.0302,
AUTHOR = {Ioana Berindan-Neagoe, Roxana Chiorean, Cornelia Braicu, Ioan Stefan Florian, Daniel Leucuta, Doinita Crisan, Adriana Cocis, Ovidiu Balacescu, Alexandru Irimie},
TITLE = {Quantitative mRNA expression of genes involved in angiogenesis, coagulation and inflammation in multiforme glioblastoma tumoral tissue versus peritumoral brain tissue: lack of correlation with clinical data},
JOURNAL = {European Cytokine Network},
VOLUME = {23},
YEAR = {2012},
NUMBER = {2},
PAGES = {45--55},
URL = {http://www.techscience.com/ECN/v23n2/65751},
ISSN = {1952-4005},
ABSTRACT = {Glioblastoma multiforme is a very aggressive brain tumor. Angiogenesis, the formation of new blood
vessels from pre-existing ones, is a process that plays an essential role in cancer development. The evolution of this
process depends upon several proangiogenic factors as well as inhibitors of angiogenesis. Coagulation and inflammation
also play an important role in tumorigenesis. Their expression is controlled by over- or under-expression
of certain genes. The objective of our study was to evaluate the expression, in tissue samples, of a set of six genes
involved in tumoral angiogenesis. The study concerned a group of 14 patients with pathologically-confirmed glioblastoma
multiforme. Samples of tumoral and peritumoral brain tissue were taken during surgery. We used RT-PCR
to evaluate the expression of six genes involved in angiogenesis: VEGF, PDGF, EPCR, TNF, ICAM-1 and CTGF.
Gene expression was calculated by comparing the values obtained for the tumoral tissue with those obtained for
the peritumoral tissue. Increased transcription of VEGF, PDGF and ICAM-1 genes were observed in glioblastoma
tumoral tissues compared with peritumoral brain tissues. Correlations were observed between transcription of the
CTGF and TNF genes (rho = 0.54, p-value = 0.05) and PDGF and ICAM-1 genes (rho = 0.54, p-value = 0.05).
Under-expression of CTGF, EPCR and TNF genes was observed in glioblastoma tumoral tissue in comparison
with peritumoral brain tissue. The association between gene expression and histopathological results (endothelial
hyperplasia, coagulation necrosis and ischemic necrosis) was evaluated. No statistically significant associations were
observed between gene expression and survival rates.},
DOI = {10.1684/ecn.2012.0302}
}