@Article{ecn.2014.0347,
AUTHOR = {Harika Atmaca, Selim Uzunoglu},
TITLE = {Anti-angiogenic effects of trabectedin (Yondelis; ET-743) on human breast cancer cells},
JOURNAL = {European Cytokine Network},
VOLUME = {25},
YEAR = {2014},
NUMBER = {1},
PAGES = {1--7},
URL = {http://www.techscience.com/ECN/v25n1/65562},
ISSN = {1952-4005},
ABSTRACT = {Trabectedin, a tetrahydroisoquinoline alkaloid derived from a Caribbean tunicate Ecteinascidia
turbinata, has been shown to have antitumor effects. In this study, we assessed the possible anti-angiogenic effects of
trabectedin on human umbilical vein endothelial cells (HUVECs) and breast cancer cell lines. An XTT cell viability
assay was used to determine cytotoxicity. A scratch assay was used to detect the migration of cells after trabectedin
treatment. Angiogenic cytokine profiles of breast cancer cell lines, before and after treatment with trabectedin, were
investigated using an angiogenesis antibody array. Changes in mRNA expression levels of VEGF were evaluated
using qRT-PCR. Trabectedin inhibited the viability of HUVECs and breast cancer cells in a concentration- and
time-dependent manner. The migration of both HUVECs and breast cancer cells was suppressed by trabectedin
treatment. Angiogenic cytokines which are known to regulate tumorigenicity and angiogenesis, such as GM-CSF,
IGFBP-2, VEGF, and uPA, were inhibited, while several anti-angiogenic cytokines such as TIMP-1 and Serpin
E1were induced in breast cancer cells. Furthermore, expression levels of VEGF mRNA were inhibited in all breast
cancer cells tested. Although additional studies are needed to elucidate the molecular mechanisms underlying the
anti-angiogenic activity of trabectedin, our results suggest that trabectedin may act as a potential anti-angiogenic
agent in breast cancer cells.},
DOI = {10.1684/ecn.2014.0347}
}