@Article{ecn.2014.0348,
AUTHOR = {Martin Kováč, Martina Vášková, Denisa Petráčková, Vendula Pelková, Ester Mejstříková, Tomáš Kalina, Markéta Žaliová, Jaroslav Weiser, Jan Starý, Ondřej Hrušák},
TITLE = {Cytokines, growth, and environment factors in bone marrow plasma of acute lymphoblastic leukemia pediatric patients},
JOURNAL = {European Cytokine Network},
VOLUME = {25},
YEAR = {2014},
NUMBER = {1},
PAGES = {8--13},
URL = {http://www.techscience.com/ECN/v25n1/65563},
ISSN = {1952-4005},
ABSTRACT = {Acute lymphoblastic leukemia (ALL) cells depend on the microenvironment of the host in vivo and do
not survive in in vitro culture. Conversely, the suppression of non-malignant tissues is one of the leading characteristics
of the course of ALL. Both the non-malignant suppression and malignant cell survival may be partly affected
by soluble factors within the bone marrow (BM) environment. Here, we aimed to identify proteins in BM plasma of
children with ALL that may contribute to ALL aggressiveness and/or the microenvironment-mediated survival of
ALL cells. LBMp (leukemic bone marrow plasma) at the time of ALL diagnosis was compared to control plasma of
bone marrow (CBMp) or peripheral blood (CPBp) using a cytokine antibody array. The cytokine antibody array
enabled simultaneous detection of 79 proteins per sample. Candidate proteins exhibiting significantly different
profiles were further analyzed and confirmed by ELISA. mRNA expression of one of the candidate proteins (TIMP1)
was studied using quantitative reverse transcriptase polymerase chain reaction (qRTPCR). The cytokine antibody
array experiments identified 23 proteins that differed significantly (p<0.05); of these, two proteins (TIMP1 and
LIF) withstood the Bonferroni correction. In contrast, little difference was observed between CBMp and CPBp.
At the diagnosis of ALL, changes in the soluble microenvironment are detectable in BM plasma. These changes
probably participate in the pathogenesis and/or result from the changes in the cell composition.},
DOI = {10.1684/ecn.2014.0348}
}