@Article{ecn.2014.0358,
AUTHOR = {Anargyros Kouris, Aikaterini Pistiki, Alexandros Katoulis, Marianna Georgitsi, Sofia Giatrakou, Evangelia Papadavid, Mihai G. Netea, Nikolaos Stavrianeas, Evangelos J. Giamarellos-Bourboulis},
TITLE = {Proinflammatory cytokine responses in patients with psoriasis},
JOURNAL = {European Cytokine Network},
VOLUME = {25},
YEAR = {2014},
NUMBER = {4},
PAGES = {63--68},
URL = {http://www.techscience.com/ECN/v25n4/65554},
ISSN = {1952-4005},
ABSTRACT = {Background: Psoriasis is one of the most common, immune-mediated, chronic inflammatory skin diseases.
Proinflammatory cytokines play an important pathogenetic role at a local level. Objective: To assess whether
the proinflammatory cytokines IL-1β, IL-6, IL-17, IL-22 and TNF-α are released systemically during psoriasis.
Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from 30 patients with psoriasis and 30 healthy
volunteers. Cytokine production was assessed in supernatants using an enzyme immunoassay after stimulation
of PBMCs with microbial stimuli. In addition, flow cytometry was used to determine the subsets of monocytes
involved and the intracellular TNF-α production in monocytes. Results: IL-17 levels were significantly higher in
the supernatants of PBMCs from psoriatic patients after stimulation with phytohemagglutinin. TNF-α production
was also significantly higher in cells from psoriatic patients after stimulation with all stimuli, as compared
with health volunteers. Similar changes were not found for the other cytokines. A statistically significant difference
was observed between patients and controls for inflammatory CD14<sup>+</sup>/CD16<sup>+</sup> monocytes (p<0.0001) and
patrolling CD14<sup>-</sup>/CD16<sup>+</sup> monocytes. Conclusion: Hyper-production of TNF-α is documented in psoriasis. These
results support the concept that there is a systemic, proinflammatory component in psoriasis.},
DOI = {10.1684/ecn.2014.0358}
}